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Rapid detection of aflatoxin B1 by dummy template molecularly imprinted polymer capped CdTe quantum dots

  • Pengqi Guo
  • Wu Yang
  • Hao Hu
  • Yitao Wang
  • Peng LiEmail author
Research Paper
  • 26 Downloads

Abstract

A novel and sensitive fluorescent sensor was synthesized for the rapid and specific recognition of aflatoxin B1 (AFB1) by our combining molecular imprinting techniques with quantum dot technology. Molecularly imprinted polymers coated CdTe quantum dots (MIP@CdTe QDs) were prepared through the Stöber method with 5,7-dimethoxycoumarin as a dummy template. 3-Aminopropyltriethoxysilane was selected as the functional monomer, and tetraethyl orthosilicate was used as the cross-linking agent. The best molar ratio of 5,7-dimethoxycoumarin to functional monomer to cross-linker was 4:20:15. The MIP@CdTe QD composites were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence spectroscopy. Under the optimum conditions, the relative fluorescence intensity of the MIP@CdTe QDs showed adequate linearity with AFB1 concentration over the range from 80 to 400 ng/g. The detection limit is 4 ng/g, according to 3s/K. Finally, the method was successfully applied to the quantitative determination of AFB1 in real samples. The spike recoveries at different spiking levels ranged from 99.20% to 101.78%, which were consistent with those measured by ultrahigh-performance liquid chromatography–mass spectrometry. The method developed for AFB1 detection lays the foundation for rapid detection of trace amounts of other exogenous harmful substances in a complicated matrix.

Keywords

CdTe quantum dots Dummy template molecularly imprinted polymers Aflatoxin B1 Fluorescent probe 

Notes

Acknowledgements

This work was supported by the Macau Science and Technology Development Fund (162/2017/A3) and the Research Committee of the University of Macau (MYRG2018-00239-ICMS and MYRG2014-00089-ICMS-QRCM).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_1708_MOESM1_ESM.pdf (289 kb)
ESM 1 (PDF 288 kb)

References

  1. 1.
    Chen AJ, Jiao X, Hu Y, Lu X, Gao W. Mycobiota and mycotoxins in traditional medicinal seeds from China. Toxins. 2015;7(10):3858–75.CrossRefGoogle Scholar
  2. 2.
    Garner RC, Miller EC, Miller JA. Liver microsomal metabolism of aflatoxin B1 to a reactive derivative toxic to Salmonella typhimurium TA 1530. Cancer Res. 1972;32(10):2058–66.Google Scholar
  3. 3.
    Li Y, Sun L, Zhao Q. Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA. Anal Bioanal Chem. 2018;410:6269–77.CrossRefGoogle Scholar
  4. 4.
    Zhao SP, Zhang D, Tan LH, Yu B, Cao W. Analysis of aflatoxins in traditional Chinese medicines: classification of analytical method on the basis of matrix variations. Sci Rep. 2016;6:30822.CrossRefGoogle Scholar
  5. 5.
    Liu SH, Chuang WC, Lam W, Jiang Z, Cheng YC. Safety surveillance of traditional Chinese medicine: current and future. Drug Saf. 2015;38(2):117–28.CrossRefGoogle Scholar
  6. 6.
    Zhang X, Liu W, Logrieco AF, Yang M, Ou-yang Z, Wang X, et al. Determination of zearalenone in traditional Chinese medicinal plants and related products by HPLC–FLD. Food Addit Contam Part A: Chem Anal Control Expo Risk Assess. 2011;28(7):885–93.CrossRefGoogle Scholar
  7. 7.
    Golge O, Hepsag F, Kabak B. Determination of aflatoxins in walnut sujuk and Turkish delight by HPLC-FLD method. Food Control. 2016;59:731–6.CrossRefGoogle Scholar
  8. 8.
    Hickert S, Gerding J, Ncube E, Hübner F, Flett B, Cramer B, et al. A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples. Mycotoxin Res. 2015;31(2):109–15.CrossRefGoogle Scholar
  9. 9.
    Liu Q, Kong W, Guo W, Yang M. Multi-class mycotoxins analysis in Angelica sinensis by ultra fast liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B. 2015;988:175–81.CrossRefGoogle Scholar
  10. 10.
    Eslami M, Mashak Z, Heshmati A, Shokrzadeh M, Mozaffari Nejad AS. Determination of aflatoxin B1 levels in Iranian rice by ELISA method. Toxin Rev. 2015;34(3):125–8.CrossRefGoogle Scholar
  11. 11.
    Turner NW, Subrahmanyam S, Piletsky SA. Analytical methods for determination of mycotoxins: a review. Anal Chim Acta. 2009;632(2):168–80.CrossRefGoogle Scholar
  12. 12.
    Li J, Zhu JJ. Quantum dots for fluorescent biosensing and bio-imaging applications. Analyst. 2013;138(9):2506–15.CrossRefGoogle Scholar
  13. 13.
    Shi J, Chan C, Pang Y, Ye W, Tian F, Lyu J, et al. A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus. Biosens Bioelectron. 2015;67:595–600.CrossRefGoogle Scholar
  14. 14.
    Kattke MD, Gao EJ, Sapsford KE, Stephenson LD, Kumar A. FRET-based quantum dot immunoassay for rapid and sensitive detection of Aspergillus amstelodami. Sensors. 2011;11(6):6396–410.CrossRefGoogle Scholar
  15. 15.
    Zhang Z, Li J, Wang X, Shen D, Chen L. Quantum dots based mesoporous structured imprinting microspheres for the sensitive fluorescent detection of phycocyanin. ACS Appl Mater Interfaces. 2015;7(17):9118–27.CrossRefGoogle Scholar
  16. 16.
    Niu M, Pham-Huy C, He H. Core-shell nanoparticles coated with molecularly imprinted polymers: a review. Microchim Acta. 2016;183(10):2677–95.CrossRefGoogle Scholar
  17. 17.
    Ren X, Chen L. Quantum dots coated with molecularly imprinted polymer as fluorescence probe for detection of cyphenothrin. Biosens Bioelectron. 2015;64:182–8.CrossRefGoogle Scholar
  18. 18.
    Chen L, Wang X, Lu W, Wu X, Li J. Molecular imprinting: perspectives and applications. Chem Soc Rev. 2016;45(8):2137–211.CrossRefGoogle Scholar
  19. 19.
    Guo P, Xu X, Chen G, Bashir K, Shu H, Ge Y, et al. On-line two dimensional liquid chromatography based on skeleton type molecularly imprinted column for selective determination of sulfonylurea additive in Chinese patent medicines or functional foods. J Pharm Biomed Anal. 2017;146:292–301.CrossRefGoogle Scholar
  20. 20.
    Qiu C, Xing Y, Yang W, Zhou Z, Wang Y, Liu H, et al. Surface molecular imprinting on hybrid SiO2-coated CdTe nanocrystals for selective optosensing of bisphenol A and its optimal design. Appl Surf Sci. 2015;345:405–17.CrossRefGoogle Scholar
  21. 21.
    Shinde S, El-Schich Z, Malakpour A, Wan W, Dizeyi N, Mohammadi R, et al. Sialic acid-imprinted fluorescent core–shell particles for selective labeling of cell surface glycans. J Am Chem Soc. 2015;137(43):13908–12.CrossRefGoogle Scholar
  22. 22.
    Li J, Fu J, Yang Q, Wang L, Wang X, Chen L. Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater. Analyst. 2018;143(15):3570–8.CrossRefGoogle Scholar
  23. 23.
    Wyszomirski M, Prus W. Molecular modelling of a template substitute and monomers used in molecular imprinting for aflatoxin B1 micro-HPLC analysis. Mol Simul. 2012;38(11):892–5.CrossRefGoogle Scholar
  24. 24.
    Wuister SF, Swart I, van Driel F, Hickey SG, de Mello Donegá C. Highly luminescent water-soluble CdTe quantum dots. Nano Lett. 2003;3(4):503–7.CrossRefGoogle Scholar
  25. 25.
    Sarwar T, Husain MA, Rehman SU, Ishqi HM, Tabish M. Multi-spectroscopic and molecular modelling studies on the interaction of esculetin with calf thymus DNA. Mol BioSyst. 2015;11(2):522–31.CrossRefGoogle Scholar
  26. 26.
    Yu J, Song N, Zhang Y-K, Zhong S-X, Wang A-J, Chen J. Green preparation of carbon dots by Jinhua bergamot for sensitive and selective fluorescent detection of Hg2+ and Fe3+. Sensors Actuators B Chem. 2015;214:29–35.CrossRefGoogle Scholar
  27. 27.
    Stöber W, Fink A, Bohn E. Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci. 1968;26(1):62–9.CrossRefGoogle Scholar
  28. 28.
    Koole R, van Schooneveld MM, Hilhorst J, de Mello Donegá C, ’t hart DC, van Blaaderen A, et al. On the incorporation mechanism of hydrophobic quantum dots in silica spheres by a reverse microemulsion method. Chem Mater. 2008;20(7):2503–12.CrossRefGoogle Scholar
  29. 29.
    Gao ZH, Lin ZZ, Chen XM, Lai ZZ, Huang ZY. Carbon dots-based fluorescent probe for trace Hg2+ detection in water sample. Sensors Actuators B Chem. 2016;222:965–71.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Pengqi Guo
    • 1
    • 2
  • Wu Yang
    • 1
  • Hao Hu
    • 1
  • Yitao Wang
    • 1
  • Peng Li
    • 1
    Email author
  1. 1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical SciencesUniversity of MacauMacauChina
  2. 2.School of Chemical EngineeringNorthwest UniversityXi’anChina

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