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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 19, pp 4861–4871 | Cite as

High-resolution and high-repetition-rate vibrational sum-frequency generation spectroscopy of one- and two-component phosphatidylcholine monolayers

  • Freeda Yesudas
  • Mark Mero
  • Janina Kneipp
  • Zsuzsanna HeinerEmail author
Research Paper
Part of the following topical collections:
  1. Young Investigators in (Bio-)Analytical Chemistry

Abstract

We present broadband vibrational sum-frequency generation (VSFG) spectra of Langmuir-Blodgett monolayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and different mixtures of them as model systems of pulmonary surfactants. The systematic study explored the dependence of the vibrational spectra as a function of surface tension and mixture ratio in various polarization combinations. The extremely short acquisition time and the high spectral resolution of our recently developed spectrometer helped minimize sample degradation under ambient conditions throughout the duration of the measurement and allowed the detection of previously unseen vibrational bands with unprecedented signal-to-noise ratio. The dramatically improved capability to record reliable vibrational spectra together with the label-free nature of the VSFG method provides direct access to native lipid structure and dynamics directly in the monolayer. The resulting data deliver quantitative information for structural analysis of multi-component phospholipid monolayers and may aid in the development of new synthetic pulmonary surfactants.

Keywords

Lung surfactants 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) Broadband vibrational sum-frequency generation spectroscopy Two-component phosphatidylcholine Langmuir-Blodgett monolayer 

Notes

Acknowledgments

We thank K. Balasubramanian (SALSA, Humboldt-Universität zu Berlin) and R. M. Iost for providing support in the sample cleaning processes.

Funding information

This project is financed by the Deutsche Forschungsgemeinschaft (DFG) project GSC 1013 SALSA. F.Y. is grateful for the support by a fellowship in SALSA. Z. H. acknowledges the funding of her Julia Lermontova fellowship by GSC 1013 SALSA.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1690_MOESM1_ESM.pdf (466 kb)
ESM 1 (PDF 466 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Freeda Yesudas
    • 1
    • 2
  • Mark Mero
    • 3
  • Janina Kneipp
    • 1
    • 2
  • Zsuzsanna Heiner
    • 1
    • 2
    Email author
  1. 1.School of Analytical Sciences AdlershofHumboldt Universität zu BerlinBerlinGermany
  2. 2.Department of ChemistryHumboldt Universität zu BerlinBerlinGermany
  3. 3.Max Born Institute for Nonlinear Optics and Short Pulse SpectroscopyBerlinGermany

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