Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone
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Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78–25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1–93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.
KeywordsLateral flow assay Fluorescent quenching Zearalenone Quantum dots
This work was supported Grant from the National Key Technology R & D Program, No. 2008BAK42B05 and Guangdong Province Key Scientific Research, No. 2013A022100031.
Compliance with ethical standards
Use of immune spleen cells for the production of monoclonal antibodies was approved by the Experimental Animal Ethics Committee of Jinan University. The Jinan University Experimental Animal Ethics Committee approved all animal experiments for preparation of anti-ZEN antibody and all experiments according to China Laboratory Animal Guidelines.
Conflicts of interest
There is no conflict of interest.
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