Simultaneous metabolic mapping of different anatomies by 1H HR-MAS chemical shift imaging
Localized information on a specimen is considered indispensable for deciphering biological activity. Magnetic resonance spectroscopy is a notable method because of its versatility; however, one limitation is the spectral quality on a static sample. This study explores an amalgamated method with two magnetic resonance experiments: high-resolution magic-angle spinning (HR-MAS) for high-quality spectral acquisition from a spinning sample and chemical shift imaging (CSI) for spatial localization. The advantage of HR-MAS CSI is its amenity for simultaneously profiling the metabolome—with good spectral data—at different spatial regions in a single experiment. Herein, 1H HR-MAS CSI (including a T2-contrast CSI) was described and performed on various food tissues and an intact organism. Different data analyses such as multivariate and quantification were explored to identify the metabolic variants in different anatomical regions and in one case, to assist in a spatial allocation. The limitation and drawback of the experiment are also discussed.
KeywordsMetabolomics HR-MAS NMR Chemical shift imaging CSI Localized metabolic profiling Metabolic mapping
We are grateful to Dr. Franck Fayon and his team (Orleans, France) for their initial assistance on the experimental setup and also to Dr. Gaspard Huber (CEA-Saclay, France) for the discussions and reviewing the manuscript.
This work was financially supported by Agence Nationale de la Recherché (ANR-16-CE11-0023-01 and ANR-12-JSV5-0005-01).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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