Fabrication of micro-patterned substrates for plasmonic sensing by piezo-dispensing of colloidal nanoparticles
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In this work we describe a very fast and flexible method for fabrication of plasmon-supporting substrates with micro-patterning capability, which is optimized for plasmonic sensing. We combined a wet chemistry approach to synthesize metallic nanoparticles with a piezo-dispensing system enabling deposition of nanoparticles on the substrates with micrometer precision. In this way, an arbitrary pattern consisting of 200 μm small spots containing plasmonic nanostructures can be produced. Patterns with various nanoparticles exhibiting different plasmonic properties were combined, and the surface density of the particles could be easily varied via their solution concentrations. We showed that under controlled conditions the dispensing process caused no aggregation of the particles and it enabled full transfer of the colloidal solutions onto the substrate. This is an important condition, which enables these substrates to be used for reliable plasmonic sensing based on monitoring the spectral shift of the nanoparticles. We demonstrated the functionality of such substrates by detection of small protein adsorption on the spots based on plasmon label-free sensing method.
KeywordsNanofabrication Plasmonic Nanoparticles Sensing Spotting
This work was financially supported by EU Era-NET/ Federal Ministry of Education and Research Germany (BMBF) projects, WaterChip (FKZ: 01DQ16009A), RA-detect (IGSTC 2015, FKZ: 01DQ16003) and RAPID (FKZ: 01DH17058). The Jenaer Biochip initiative (JBCI) is gratefully acknowledged for providing access to the GeSIM microarray spotter.
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Conflict of interest
The authors declare that they have no competing interests.
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