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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 6, pp 1261–1271 | Cite as

Cost-effective imprinting to minimize consumption of template in room-temperature ionic liquid for fast purification of chlorogenic acid from the extract of E. ulmoides leaves

  • Ya Kun Sun
  • Guang-Ying Sun
  • Man Jia
  • Jian Yang
  • Zhao-Sheng Liu
  • Yan-Ping HuangEmail author
  • Haji Akber AisaEmail author
Research Paper
  • 17 Downloads

Abstract

One of the main challenges in large-scale applications of molecularly imprinted polymers (MIPs) is the significant amount of template needed in polymer preparation. A new strategy based on room-temperature ionic liquids (RTILs) was suggested to solve this problem by reducing the amount of template in the polymerization recipe. The MIP was synthesized with a mixture of dimethyl sulfoxide and RTIL (1-butyl-3-methylimidazolium tetrafluoroborate) as porogen, in which chlorogenic acid (CGA) was used as template, 4-vinylpyridine (4-VP) as functional monomer, and ethylene glycol dimethacrylate (EDMA) as cross-linker. The influence of polymerization variables, including CGA concentrations, and the ratio of 4-VP to EDMA on imprinting effect were investigated comprehensively. Moreover, the properties involving the column permeability, the number of binding sites, and the polymer morphology of the CGA-MIP monoliths were studied thoroughly. The MIP monolith had an excellent column permeability (1.53 × 10−13 m2) and allowed an ultra-fast on-line SPE, which dramatically shortens the separation time (< 10 min) and improves the separation efficiency. At high flow velocity (5.0 mL min−1), 50 μL of the extract from Eucommia ulmoides leaves can be loaded directly on the CGA-MIP monoliths and CGA with high purity can be obtained with a recovery of 89.01 ± 0.05%. As a conclusion, the resulting RTIL-induced approach of preparing MIP may be an effective tool in fabricating MIP in a low-cost way.

Graphical abstract

Keywords

Molecularly imprinted polymer Chlorogenic acid Monolith On-line solid-phase extraction Room-temperature ionic liquid 

Notes

Funding information

This work was supported by the West Light Foundation of the Chinese Academy of Sciences (Grant No. 2017-XBYJRC-002).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1559_MOESM1_ESM.pdf (383 kb)
ESM 1 (PDF 383 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ya Kun Sun
    • 1
    • 2
    • 3
  • Guang-Ying Sun
    • 1
    • 2
  • Man Jia
    • 1
    • 2
    • 3
  • Jian Yang
    • 1
    • 2
    • 3
  • Zhao-Sheng Liu
    • 1
    • 2
  • Yan-Ping Huang
    • 1
    • 2
    Email author
  • Haji Akber Aisa
    • 1
    • 2
    Email author
  1. 1.Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiChina
  2. 2.State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiChina
  3. 3.University of Chinese Academy of SciencesBeijingChina

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