Utilization of atmospheric solids analysis probe mass spectrometry for analysis of fatty acids on seed surface
Atmospheric solids analysis probe mass spectrometry (ASAP-MS) was used for the first time for direct surface analysis of plant material. It can be readily used for surface analysis of whole and intact pea seeds and their seed coats, and for the study of the profile of fatty acids on the outer surface. Furthermore, ASAP-MS in combination with multivariate statistics allowed classification of pea genotypes with respect to physical dormancy and investigation of related biological markers. Hexacosanoic and octacosanoic acids were suggested to be important markers likely influencing water transport through the seed coat into the embryo (with the highest significance for dormant L100 genotype). ASAP-MS provided higher selectivity and better signal of fatty acids compared to (MA)LDI-MS (laser desorption ionization mass spectrometry either matrix free or matrix assisted) providing on the other hand spatial distribution information and results obtained by both methods are mutually supportive. The developed ASAP-MS method and obtained results can be widely utilized in biological, food, and agricultural research.
KeywordsAtmospheric solids analysis probe Mass spectrometry Fatty acid Pea Physical dormancy Legume seed
This study received the support from Operational Programme Research, Development and Education – European Regional Development Fund, project no. CZ.02.1.01/0.0/0.0/16_019/0000754 and Palacký University Olomouc (IGA_PrF_2018_027).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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