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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 6, pp 1193–1202 | Cite as

A novel coating method for CE capillary using carboxymethyl-β-cyclodextrin-modified magnetic microparticles as stationary for electrochromatography enantioseparation

  • Xiaodong Sun
  • Jie Guo
  • Tao Yu
  • Yingxiang DuEmail author
  • Zijie Feng
  • Shiyuan Zhao
  • Zhifeng Huang
  • Jie Liu
Research Paper

Abstract

Magnetic microparticles (MMPs) have been extensively studied and aroused considerable interest in separation science owing to their superior characteristics. In this paper, a novel coated capillary with carboxymethyl-β-cyclodextrin-functionalized magnetic microparticles (CD-MMPs) as stationary phase was constructed and then applied to establish an open-tubular capillary electrochromatography enantioseparation system. The preparation of the CD-MMP-coated open-tubular column was very convenient because the coating of the magnetic microparticles onto the capillary column could be easily manipulated by an external magnetic field. The preparation conditions of the coated capillary such as magnetic field intensity and coating time are discussed in detail. The new constructed CD-MMP capillary system was applied to separate enantiomers of several racemic drugs. Compared to the uncoated capillary system, obviously preferable separations of tested enantiomers were obtained. Several important parameters affecting the enantioseparation, such as CM-β-CD concentration, running buffer pH, organic solvent, and applied voltage, were systematically optimized. Furthermore, satisfactory repeatability and chemical stability of this new CD-MMP capillary system were achieved in the experiment.

Graphical abstract

Keywords

Capillary electrochromatography Enantioseparation Carboxymethyl-β-cyclodextrin-functionalized magnetic microparticles Magnetic coating 

Abbreviations

AML

Amlodipine

APTES

3-Aminopropyltriethoxysilane

ATE

Atenolol

CD-MMPs

Carboxymethyl-β-cyclodextrin-modified APTES–Fe3O4 magnetic microparticles

CE

Capillary electrophoresis

CEC

Capillary electrochromatography

CHL

Chlortrimeton

CM-β-CD

Carboxymethyl-β-cyclodextrin

EDC

N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride

EOF

Electroosmotic flow

GC

Gas chromatography

HPLC

High-performance liquid chromatography

MMPs

Magnetic microparticles

MPs

Microparticles

NHS

N-Hydroxysuccinimide

NPs

Nanoparticles

OFL

Ofloxacin

OT-CEC

Open-tubular capillary electrochromatography

PRO

Propranolol

SFC

Supercritical fluid chromatography

TRO

Tropicamide

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.BK20150697) and the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

216_2018_1545_MOESM1_ESM.pdf (998 kb)
ESM 1 (PDF 997 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Analytical ChemistryChina Pharmaceutical UniversityNanjingChina
  2. 2.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education)China Pharmaceutical UniversityNanjingChina
  3. 3.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingChina

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