An integrated proteomic and glycoproteomic study for differences on glycosylation occupancy in rheumatoid arthritis
Rheumatoid arthritis (RA) is an autoimmune disease in which certain immune cells are dysfunctional and attack their own healthy tissues. There has been great difficulty in finding an accurate and efficient method for the diagnosis of early-stage RA. The present shortage of diagnostic methods leads to the rough treatments of the patients in the late stages, such as joint removing. Nowadays, there is an increasing focus on glyco-biomarkers discovery for malicious disease via MS-based strategy. In this study, we present an integrated proteomics and glycoproteomics approach to uncover the pathological changes of some RA-related glyco-biomarkers and glyco-checkpoints involved in the RA onset. Among 39 distinctly expressive N-glycoproteins, 27 N-glycoproteins were discovered with over twofold expression significances. On the other hand, 13 proteins have been distinguished with significant differences in 53 distinctly expressed proteins identified in this study. Such an integrated approach will provide a comprehensive strategy for new potential glyco-biomarkers and checkpoints discovery in rheumatoid arthritis.
KeywordsBiomarker Label-free quantification Mass spectrometry Rheumatoid arthritis
We sincerely thank Georgia Research Alliance (GRA) and Georgia State University for purchasing the analytical instrument used in this research.
Compliance with ethical standards
The study was approved by the Ethics Committee of Peking University People’s Hospital (Approval No. 2015 PHB 219-01). All participants of this study provided informed consent for participation in this study.
Conflict of interest
The authors declare that they have no conflicts of interest.
- 5.Burska A, Boissinot M, Ponchel F. Cytokines as biomarkers in rheumatoid arthritis. Mediat Inflamm. 2014;2014:545493.Google Scholar
- 9.Varki ACR, Esko J, Freeze H, Hart G, Marth J. Essentials of glycobiology. New York: Cold spring harbor laboratory press; 1999.Google Scholar
- 30.Barker PE, Wagner PD, Stein SE, Bunk DM, Srivastava S, Omenn GS. Standards for plasma and serum proteomics in early cancer detection: a needs assessment report from the national institute of standards and technology--National Cancer Institute standards, methods, assays, reagents and technologies workshop, August 18-19, 2005. Clin Chem. 2006;52(9):1669–74.CrossRefGoogle Scholar
- 42.Janeway CA Jr, Travers P, Walport M, Shlomchik MJ. The complement system and innate immunity. Immunobiology: the immune system in health and disease. 5th ed. New York: Garland Science; 2001.Google Scholar