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A novel switchable fluorescent sensor for facile and highly sensitive detection of alkaline phosphatase activity in a water environment with gold/silver nanoclusters

  • Xiaoyan Wang
  • Zhenjiang Liu
  • Wanying Zhao
  • Jianfan SunEmail author
  • Bin Qian
  • Xinwei Wang
  • Huawei Zeng
  • Daolin Du
  • Jinsheng DuanEmail author
Research Paper
  • 102 Downloads

Abstract

A novel fluorescent sensor based on bovine serum albumin stabilized gold/silver nanoclusters (BSA-Au/Ag NCs) was developed for sensitive and facile detection of alkaline phosphatase (ALP) activity. For this fluorescent sensor, ascorbic acid 2-phosphate (AAP) was decomposed into ascorbic acid (AA) and phosphate by catalysis with ALP. The initial red fluorescence of the BSA-Au/Ag NCs was effectively quenched by KMnO4 and then the fluorescence was recovered by addition of AA. The mechanism of interaction between BSA-Au/Ag NCs and KMnO4 and AA was studied with use of the fluorescence lifetime and UV-vis absorption spectra. The results indicated that the oxidation/reduction modulated by KMnO4/AA led to surface structure destruction/restoration of the BSA-Au/Ag NCs, resulting in fluorescence quenching/recovery. The proposed fluorescence-based method based on a dark background was used to detect ALP and had excellent sensitivity, with a detection limit of 0.00076 U/L. Moreover, the method was applied to the determination of added analytes, with satisfactory recoveries (97.0–105.0 %). In a simulated eutrophic water body, this method successfully detected ALP in actual water samples and could monitor the dynamic changes of ALP activity through visual observation. More importantly, the proposed fluorescent sensor not only has the advantages of simple operation and high sensitivity but has also been successfully used on filter paper to establish a rapid and visual test paper for ALP.

Keywords

Au/Ag nanoclusters stabilized with bovine serum albumin Alkaline phosphate activity Fluorescence quenching/recovery 

Notes

Acknowledgements

This work was supported by the State Key Research Development Program of China (2017YFC1200103), the National Natural Science Foundation of China (NSFC-31200317, 31570414), the Senior Talent Scientific Research Initial Funding Project of Jiangsu University (11JDG1147, 14JDG051), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment.

Compliance with ethical standards

All authors contributed to the work, read the manuscript, and agreed to be listed as an author.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2018_1514_MOESM1_ESM.pdf (1.8 mb)
ESM 1 (PDF 1.82 MB)

References

  1. 1.
    Dodds WK, Smith VH. Nitrogen, phosphorus, and eutrophication in streams. Inland Waters. 2016;6:155–64.CrossRefGoogle Scholar
  2. 2.
    Dodds WKK, Welch EB. Establishing nutrient criteria in streams. J N Am Benthol Soc. 2000;19:186–96.CrossRefGoogle Scholar
  3. 3.
    Smith VH. Eutrophication of freshwater and coastal marine ecosystems: a global problem. Environ Sci Pollut Res. 2003;10:126–39.CrossRefGoogle Scholar
  4. 4.
    Ardón M, Montanari S, Morse JL, Doyle MW, Bernhardt ES. Phosphorus export from a restored wetland ecosystem in response to natural and experimental hydrologic fluctuations. J Geophys Res Biogeosci. 2015;115:2532–4.Google Scholar
  5. 5.
    Davis TW, Bullerjahn GS, Tuttle T, Mckay RM, Watson SB. Effects of increasing nitrogen and phosphorus concentrations on phytoplankton community growth and toxicity during Planktothrix blooms in Sandusky Bay, Lake Erie. Environ Sci Technol. 2015;49:7197–207.CrossRefGoogle Scholar
  6. 6.
    Qin BQ, Gao G, Zhu GW, Zhang YL, Song YZ, Tang XM, et al. Lake eutrophication and its ecosystem response. Sci Bull. 2013;58:961–70.CrossRefGoogle Scholar
  7. 7.
    Liu W, Zhang Q, Liu G. Lake eutrophication associated with geographic location, lake morphology and climate in China. Hydrobiologia. 2010;644:289–99.CrossRefGoogle Scholar
  8. 8.
    Carpenter SR, Ludwig D, Brock WA. Management of eutrophication for lakes subject to potentially irreversible change. Ecol Appl. 1999;9:751–71.CrossRefGoogle Scholar
  9. 9.
    Dodds WK, Bouska WW, Eitzmann JL, Pilger TJ, Pitts KL, Riley AJ, et al. Eutrophication of U.S. freshwaters: analysis of potential economic damages. Environ Sci Technol. 2009;43:12–9.CrossRefGoogle Scholar
  10. 10.
    Ahlgren G. Phosphorus as growth-regulating factor relative to other environmental factors in cultured algae. Hydrobiologia. 1988;170:191–210.CrossRefGoogle Scholar
  11. 11.
    Spears BM, Carvalho L, Dudley B, May L. Variation in chlorophyll a to total phosphorus ratio across 94 UK and Irish lakes: Implications for lake management. J Environ Manage. 2013;115:287–94.CrossRefGoogle Scholar
  12. 12.
    Jarvie HP, Sharpley AN, Scott JT, Haggard BE, Bowes MJ, Massey LB. Within-river phosphorus retention: accounting for a missing piece in the watershed phosphorus puzzle. Environ Sci Technol. 2012;46:13284–92.CrossRefGoogle Scholar
  13. 13.
    Ding W, Zhu R, Hou L, Wang Q. Matrix-bound phosphine, phosphorus fractions and phosphatase activity through sediment profiles in Lake Chaohu, China. Environ Sci Process Impacts. 2014;16:1135–44.CrossRefGoogle Scholar
  14. 14.
    May L, Spears BM, Dudley BJ, Hattonellis TW. The importance of nitrogen limitation in the restoration of Llangorse Lake, Wales, UK. J Environ Monit. 2010;12:338–46.CrossRefGoogle Scholar
  15. 15.
    Lewis WM, Wurtsbaugh WA, Paerl HW. Rationale for control of anthropogenic nitrogen and phosphorus to reduce eutrophication of inland waters. Environ Sci Technol. 2011;45:10300–5.CrossRefGoogle Scholar
  16. 16.
    Janssen EM, Mcneill K. Environmental photoinactivation of extracellular phosphatases and the effects of dissolved organic matter. Environ Sci Technol. 2015;49:889–96.CrossRefGoogle Scholar
  17. 17.
    Rose C, Axler RP. Uses of alkaline phosphatase activity in evaluating phytoplankton community phosphorus deficiency. Hydrobiologia. 1997;361:145–56.CrossRefGoogle Scholar
  18. 18.
    Halawa MI, Gao W, Saqib M, Kitte SA, Wu F, Xu G. Sensitive detection of alkaline phosphatase by switching on gold nanoclusters fluorescence quenched by pyridoxal phosphate. Biosens Bioelectron. 2017;95:8–14.CrossRefGoogle Scholar
  19. 19.
    Vivas A, Marulanda A, Gómez M, Barea JM, Azcón R. Physiological characteristics (SDH and ALP activities) of arbuscular mycorrhizal colonization as affected by Bacillus thuringiensis inoculation under two phosphorus levels. Soil Biol Biochem. 2003;35:987–96.CrossRefGoogle Scholar
  20. 20.
    Labry C, Delmas D, Herbland A. Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters (Bay of Biscay). J Exp Mar Biol Ecol. 2005;318:213–25.CrossRefGoogle Scholar
  21. 21.
    Hu Q, Zhou B, Dang P, Li L, Kong J, Zhang X. Facile colorimetric assay of alkaline phosphatase activity using Fe(II)-phenanthroline reporter. Anal Chim Acta. 2017;950:170–7.CrossRefGoogle Scholar
  22. 22.
    Schoenau E, Herzog KH, Boehles HJ. Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates. Clin Chem. 1986;32:816–8.PubMedGoogle Scholar
  23. 23.
    Healey FP, Hendzel LL. Fluorometric measurement of alkaline phosphatase activity in algae. Freshwater Biol. 2010;9:429–39.CrossRefGoogle Scholar
  24. 24.
    Ruan C, Wang W, Gu B. Detection of alkaline phosphatase using surface-enhanced Raman spectroscopy. Anal Chem. 2006;78:3379–84.CrossRefGoogle Scholar
  25. 25.
    Zhang H, Ma X, Hu S, Lin Y, Guo L, Qiu B, et al. Highly sensitive visual detection of Avian Influenza A (H7N9) virus based on the enzyme-induced metallization. Biosens Bioelectron. 2016;79:874–80.CrossRefGoogle Scholar
  26. 26.
    Shen C, Li X, Rasooly A, Guo L, Zhang K, Yang M. A single electrochemical biosensor for detecting the activity and inhibition of both protein kinase and alkaline phosphatase based on phosphate ions induced deposition of redox precipitates. Biosens Bioelectron. 2016;85:220–5.CrossRefGoogle Scholar
  27. 27.
    Gong H, Little G, Cradduck M, Draney DR, Padhye N, Olive DM. Alkaline phosphatase assay using a near-infrared fluorescent substrate merocyanine 700 phosphate. Talanta. 2011;84:941–6.CrossRefGoogle Scholar
  28. 28.
    Gu X, Zhang G, Wang Z, Liu W, Xiao L, Zhang D. A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules. Analyst. 2013;138:2427–31.CrossRefGoogle Scholar
  29. 29.
    Lin S, Liu S, Ye F, Xu L, Zeng W, Wang L, et al. Sensitive detection of DNA by hyperbranched diketopyrrolopyrrole-based conjugated polyelectrolytes. Sens Actuators B. 2013;182:176–83.CrossRefGoogle Scholar
  30. 30.
    Qian Z, Chai L, Tang C, Huang Y, Chen J, Feng H. Carbon quantum dots-based recyclable real-time fluorescence assay for alkaline phosphatase with adenosine triphosphate as substrate. Anal Chem. 2015;87:2966–73.CrossRefGoogle Scholar
  31. 31.
    Freeman R, Finder T, Gill R, Willner I. Probing protein kinase (CK2) and alkaline phosphatase with CdSe/ZnS quantum dots. Nano Lett. 2010;10:2192–6.CrossRefGoogle Scholar
  32. 32.
    Zhang X, Deng J, Xue Y, Shi G, Zhou T. Stimulus response of Au-NPs@GMP-Tb core-shell nanoparticles: toward colorimetric and fluorescent dual-mode sensing of alkaline phosphatase activity in algal blooms of a freshwater lake. Environ Sci Technol. 2016;50:847–55.CrossRefGoogle Scholar
  33. 33.
    Tong YJ, Yu LD, Wu LL, Cao SP, Liang RP, Zhang L, et al. Aggregation-induced emission of luminol: a novel strategy for fluorescence ratiometric detection of ALP and As(V) with high sensitivity and selectivity. Chem Commun. 2018;54:7487–90.CrossRefGoogle Scholar
  34. 34.
    Zhang N, Si Y, Sun Z, Chen L, Li R, Qiao Y, et al. Rapid, selective, and ultrasensitive fluorimetric analysis of mercury and copper levels in blood using bimetallic gold–silver nanoclusters with “silver effect”-enhanced red fluorescence. Anal Chem. 2014;86:11714–21.CrossRefGoogle Scholar
  35. 35.
    Cheng T, Xu Y, Zhang S, Zhu W, Qian X, Duan L. A highly sensitive and selective OFF-ON fluorescent sensor for cadmium in aqueous solution and living cell. J Am Chem Soc. 2008;130:16160–1.CrossRefGoogle Scholar
  36. 36.
    Wang X-Y, Zhu G-B, Liu Z-J, Pan C-G, Hu W-J, Zhao W-Y, et al. A novel ratiometric fluorescent probe for the detection of uric acid in human blood based on H2O2-mediated fluorescence quenching of gold/silver nanoclusters. Talanta. 2019;191:46–53.CrossRefGoogle Scholar
  37. 37.
    Yan X, Song Y, Wu X, Zhu C, Su X, Du D, et al. Oxidase-mimicking activity of ultrathin MnO2 nanosheets in colorimetric assay of acetylcholinesterase activity. Nanoscale. 2017;9:2317–23.CrossRefGoogle Scholar
  38. 38.
    Jin L, Shang L, Guo S, Fang Y, Wen D, Wang L, et al. Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose. Biosens Bioelectron. 2011;26:1965–9.CrossRefGoogle Scholar
  39. 39.
    Vericat C, Vela ME, Benitez G, Carro P, Salvarezza RC. Self-assembled monolayers of thiols and dithiols on gold: new challenges for a well-known system. Chem Soc Rev. 2010;39:1805–34.CrossRefGoogle Scholar
  40. 40.
    Chen X, Baker GA. Cholesterol determination using protein-templated fluorescent gold nanocluster probes. Analyst. 2013;138:7299–302.CrossRefGoogle Scholar
  41. 41.
    Luo D, Smith SW, Anderson BD. Kinetics and mechanism of the reaction of cysteine and hydrogen peroxide in aqueous solution. J Pharm Sci. 2005;94:304–16.CrossRefGoogle Scholar
  42. 42.
    Long Q, Fang A, Wen Y, Li H, Zhang Y, Yao S. Rapid and highly-sensitive uric acid sensing based on enzymatic catalysis-induced upconversion inner filter effect. Biosens Bioelectron. 2016;86:109–14.CrossRefGoogle Scholar
  43. 43.
    Wei H, Chen C, Han B, Wang E. Enzyme colorimetric assay using unmodified silver nanoparticles. Anal Chem. 2008;80:7051–5.CrossRefGoogle Scholar
  44. 44.
    Zhao W, Chiuman W, Lam JC, Brook MA, Li Y. Simple and rapid colorimetric enzyme sensing assays using non-crosslinking gold nanoparticle aggregation. Chem Commun. 2007;36:3729–31.CrossRefGoogle Scholar
  45. 45.
    Deng J, Yu P, Wang Y, Mao L. Real-time ratiometric fluorescent assay for alkaline phosphatase activity with stimulus responsive infinite coordination polymer nanoparticles. Anal Chem. 2015;87:3080–6.CrossRefGoogle Scholar
  46. 46.
    Liu S, Pang S, Na W, Su X. Near-infrared fluorescence probe for the determination of alkaline phosphatase. Biosens Bioelectron. 2014;55:249–54.CrossRefGoogle Scholar
  47. 47.
    Zhang W, Ying G, Li Y, Zhang Q, Hu Z, Zhang Y, et al. Polyphosphoric acid-induced perylene probe self-assembly and label-free fluorescence turn-on detection of alkaline phosphatase. Anal Bioanal Chem. 2016;409:1–6.Google Scholar
  48. 48.
    Goggins S, Naz C, Marsh BJ, Frost CG. Ratiometric electrochemical detection of alkaline phosphatase. Chem Commun. 2014;51:561–4.CrossRefGoogle Scholar
  49. 49.
    Li FS, Zhang YL, Li XB, Li BL, Liu YF. Biosensor of alkaline phosphatase based on non-fluorescent FRET of Eu3+-doped oxide nanoparticles and phosphorylated peptide labeled with cyanine dye. Anal Bioanal Chem. 2017;409:1–10.CrossRefGoogle Scholar
  50. 50.
    Zhang Y, Li Y, Zhang C, Zhang Q, Huang X, Yang M, et al. Fluorescence turn-on detection of alkaline phosphatase activity based on controlled release of PEI-capped Cu nanoclusters from MnO2 nanosheets. Anal Bioanal Chem. 2017;409:1–8.CrossRefGoogle Scholar
  51. 51.
    Zhang X, Deng J, Xue Y, Shi G, Zhou T. Stimulus response of Au-NPs@GMP-Tb core-shell nanoparticles: toward colorimetric and fluorescent dual mode sensing of alkaline phosphatase activity in algal bloom of freshwater lake. Environ Sci Technol. 2016;50:847–55.CrossRefGoogle Scholar
  52. 52.
    Gage MA, Gorham E. Alkaline phosphatase activity and cellular phosphorus as an index of the phosphorus status of phytoplankton in Minnesota lakes. Freshwater Biol. 2010;15:227–33.CrossRefGoogle Scholar
  53. 53.
    Parolo C, Merkoçi A. Paper-based nanobiosensors for diagnostics. Chem Soc Rev. 2012;42:450–7.CrossRefGoogle Scholar
  54. 54.
    Cate DM, Adkins JA, Mettakoonpitak J, Henry CS. Recent developments in paper-based microfluidic devices. Anal Chem. 2015;87:19–41.CrossRefGoogle Scholar
  55. 55.
    Vishat SK, Luppa PB, Yeo LY, Aydogan O, Luong JHT. Emerging technologies for next-generation point-of-care testing. Trends Biotechnol. 2015;33:692–705.CrossRefGoogle Scholar
  56. 56.
    Vashist SK, Mudanyali O, Schneider EM, Zengerle R, Ozcan A. Cellphone-based devices for bioanalytical sciences. Anal Bioanaly Chem. 2014;406:3263–77.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoyan Wang
    • 1
  • Zhenjiang Liu
    • 1
  • Wanying Zhao
    • 1
  • Jianfan Sun
    • 1
    Email author
  • Bin Qian
    • 1
  • Xinwei Wang
    • 1
  • Huawei Zeng
    • 2
  • Daolin Du
    • 1
  • Jinsheng Duan
    • 3
    Email author
  1. 1.School of the Environment and Safety EngineeringJiangsu UniversityZhenjiangChina
  2. 2.College of Life SciencesHuaibei Normal UniversityHuaibeiChina
  3. 3.Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences, Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei)Ministry of AgricultureHefeiChina

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