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A bi-end injection capillary electrophoresis method for simultaneous determination of 37 cations and anions in beers

  • Chao Guo
  • Zhenpeng Guo
  • Yi Chen
Research Paper
  • 42 Downloads
Part of the following topical collections:
  1. New Insights into Analytical Science in China

Abstract

Capillary electrophoresis (CE) is excellent at separating all the ions in a sample but is rarely used as a result of its detection issue and easy loss of very fast ions by common one-end injection methods. Herein we propose a newly developed method aimed at simultaneous determination of positive and negative ions with a home-made CE device, featuring bi-end injection and contactless conductivity detection at the middle. By simply using 2.5 M acetic acid as a running buffer, the method can separate 37 ions (3 inorganic anions, 8 inorganic cations, 10 biogenic amines, and 16 amino acids) per run, with linearity between 10 and 2000 μM (R2 > 0.99), limit of detection of 1.0–16.6 μM, and limit of quantification of 2.3–31.7 μM. The recovery measured by spiking standards into samples at high, middle, and low levels was between 73% and 110%. The intra- and interday repeatability of the 37 analytes ranged from 0.69% to 8.97% and from 0.68% to 11.04%, respectively. The proposed method was evaluated by analysis of 21 beers and, in addition to acquiring the concentration information, the brands of the tested beers were distinguished. This method is of high throughput, fast, and cost-effective. It could be a promising tool for ionomic analysis.

Graphical abstract

Keywords

Capillary electrophoresis Beer ions Biogenic amines Amino acids Brand differentiation 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21727809, 21475136, 21235007 & 21621062) and Chinese Academy of Sciences (QYZDJ-SSW-SLH034).

Compliance with ethical standards

Conflict of interest

We declare that there is no conflict of interest in this article.

Supplementary material

216_2018_1507_MOESM1_ESM.pdf (333 kb)
ESM 1 (PDF 333 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of ChemistryChinese Academy of SciencesBeijingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Beijing National Laboratory for Molecular SciencesBeijingChina

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