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Complementarity of molecular and elemental mass spectrometric imaging of Gadovist in mouse tissues

  • Sabrina Trog
  • Ahmed H. El-Khatib
  • Sebastian Beck
  • Marcus R. Makowski
  • Norbert Jakubowski
  • Michael W. Linscheid
Research Paper
Part of the following topical collections:
  1. Elemental and Molecular Imaging by LA-ICP-MS

Abstract

Drug biodistribution analyses can be considered a key issue in pharmaceutical discovery and development. Here, mass spectrometric imaging can be employed as a powerful tool to investigate distributions of drug compounds in biologically and medically relevant tissue sections. Both matrix-assisted laser desorption ionization–mass spectrometric imaging as molecular method and laser ablation inductively coupled plasma–mass spectrometric imaging as elemental detection method were applied to determine drug distributions in tissue thin sections. Several mouse organs including the heart, kidney, liver, and brain were analyzed with regard to distribution of Gadovist, a gadolinium-based contrast agent already approved for clinical investigation. This work demonstrated the successful detection and localization of Gadovist in several organs. Furthermore, the results gave evidence that gadolinium-based contrast agents in general can be well analyzed by mass spectrometric imaging methods. In conclusion, the combined application of molecular and elemental mass spectrometry could complement each other and thus confirm analytical results or provide additional information.

Keywords

Matrix-assisted laser desorption ionization–mass spectrometry imaging (MALDI-MSI) Laser ablation inductively coupled plasma–mass spectrometry imaging (LA-ICP-MSI) Gadolinium-based contrast agents (GBCAs) 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures were approved by the guidelines and regulations of the Federation of Laboratory Animal Science Associations (FELASA) and the local guidelines and provisions for implementation of the Animal Welfare Act. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

216_2018_1477_MOESM1_ESM.pdf (2.9 mb)
ESM 1 (PDF 3015 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sabrina Trog
    • 1
  • Ahmed H. El-Khatib
    • 1
    • 2
  • Sebastian Beck
    • 1
  • Marcus R. Makowski
    • 3
  • Norbert Jakubowski
    • 2
  • Michael W. Linscheid
    • 1
  1. 1.Department of ChemistryHumboldt-Universität zu BerlinBerlinGermany
  2. 2.Bundesanstalt für Materialforschung und –prüfungBerlinGermany
  3. 3.Department of RadiologyCharité – Universitätsmedizin BerlinBerlinGermany

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