Analytical and Bioanalytical Chemistry

, Volume 411, Issue 2, pp 507–515 | Cite as

Carboxyl Fe3O4 magnetic nanoparticle-based SPE and HPLC method for the determination of six tetracyclines in water

  • Yi Yang
  • Shuo Yin
  • Danni Yang
  • Yang Jiang
  • Yongxin Li
  • Chen Zhou
  • Chengjun SunEmail author
Research Paper


A novel carboxyl Fe3O4 magnetic nanoparticle-based solid-phase extraction combined with high-performance liquid chromatography was developed for the analysis of oxytetracycline, tetracycline, demeclocycline, metacycline, chlortetracycline, and doxycycline in water samples. Driven by the electrostatic interaction and the strong chelation between tetracyclines and iron ions, tetracyclines in samples were adsorbed onto the adsorbents. The adsorbed analytes were subsequently eluted with oxalic acid and separated with a C18 column under gradient condition with a mobile phase consisting of methanol, acetonitrile, and oxalic acid at a flow rate of 0.5 mL/min. The detection was performed at variable ultraviolet wavelengths. Under optimized conditions, the developed method gave an enrichment factor of 33.3, linearity ranges of 5.00–1000 μg/L, detection limits of (2.86–5.19) × 10-2 μg/L, quantification limits of (9.54–17.3) × 10-2 μg/L, recoveries of 76.2–98.0%, and intra- and inter-day RSDs of 0.132–15.5% and 2.28–14.5% for these tetracyclines. The established method was successfully applied for the determination of these six tetracyclines in tap water, river water, pond water, and lake water samples.

Graphical abstract


Tetracyclines Magnetic nanoparticles Solid-phase extraction High-performance liquid chromatography Water 



This work was supported by the Fundamental Research Funds for the Central Universities of China [grant number 2012017yjsy206].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1475_MOESM1_ESM.pdf (982 kb)
ESM 1 (PDF 982 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.West China School of Public HealthSichuan UniversityChengduChina
  2. 2.Sichuan Center for Disease Control and PreventionChengduChina

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