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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 2, pp 387–393 | Cite as

Cucurbit[8]uril-assisted peptide assembly for feasible electrochemical assay of histone acetyltransferase activity

  • Xiangyang Miao
  • Yang Wang
  • Zhun Gu
  • Dongsheng Mao
  • Limin NingEmail author
  • Ya CaoEmail author
Research Paper

Abstract

Accumulating findings demonstrate the importance of histone acetyltransferases (HATs) in regulating the acetylation of histones and reveal that their aberrant catalytic activities are involved in the occurrence and progress of numerous diseases. Herein, a feasible electrochemical method is proposed to assay the activity of HAT. The critical elements of the assay method are the hindrance of HAT-catalyzed acetylation against carboxypeptidase Y-catalyzed digestion and cucurbit[8]uril-assisted peptide assembly, which may recruit peptide-templated silver nanoparticles onto the electrode surface, producing significant electrochemical signals. Taking p300 as a model HAT, the assay method is validated to exhibit desirable selectivity, reproducibility, and usability in inhibitor analysis, and allow absolute activity determination in a linear range from 0.1 to 50 nM with a detection limit of 0.055 nM, which is lower than those of previous reports. Therefore, this work may provide an effective tool for HAT activity assay, which will be of great potential in HAT-related fundamental research, disease diagnosis, and drug development in the future.

Graphical abstract

Keywords

Histone acetyltransferase p300 Cucurbit[8]uril Peptide assembly Peptide-templated silver nanoparticles Electrochemical assay 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 81503463), the Science and Technology Planning Project of Taicang (TC2018JC04) and the Natural Science Research Projects of Universities in Jiangsu Province (Project No. 16KJB430037).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1445_MOESM1_ESM.pdf (240 kb)
ESM 1 (PDF 240 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical Science and TechnologySuzhou Chien-shiung Institute of TechnologyTaicangChina
  2. 2.College of Medicine and Life SciencesNanjing University of Chinese MedicineNanjingChina
  3. 3.Center for Molecular Recognition and Biosensing, School of Life SciencesShanghai UniversityShanghaiChina

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