Analytical and Bioanalytical Chemistry

, Volume 410, Issue 27, pp 7103–7112 | Cite as

A fluorescent material for the detection of chlortetracycline based on molecularly imprinted silica–graphitic carbon nitride composite

  • Shengnan Xu
  • Jie DingEmail author
  • Ligang ChenEmail author
Research Paper


A new fluorescent probe based on graphitic carbon nitride (g-C3N4) combined with molecularly imprinted silica was successfully fabricated and used to selectively recognize chlortetracycline (CTC). The g-C3N4 used in this study has the characteristics of low toxicity and high chemical stability. This synthetic composite was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, UV spectroscopy, X-ray diffraction, and fluorescence spectroscopy. The material was used to detect CTC by the fluorescence quenching technique. The fluorescence quenching was due to g-C3N4 and the benzene ring of CTC through ππ electron donor–acceptor interaction and electrostatic force. Hydrogen bonds formed between CTC and 3-aminopropyltriethoxysilane during the polymerization process. Eventually, a considerable amount of selective recognition holes were formed in the composite material and could specifically recognize the template molecule CTC. In addition, the probe strategy was successfully applied to milk analysis, and the recoveries ranged from 90.1% to 95.7%, with relative standard deviations of 1.8–2.8%; the detection limit for CTC was 8 ng mL-1. The results indicate that this method combined the sensitivity of fluorescence detection with the excellent selectivity of a molecularly imprinted polymer. The new material can be widely used in the detection of dairy products.

Graphical abstract

Schematic of synthesis of the MIP-capped g-C3N4 by sol-gel reaction


Graphitic carbon nitride Molecularly imprinted ππ electron donor–acceptor interaction Chlortetracycline 



This work was supported by the Fundamental Research Funds for the Central Universities (no. 2572017EB08), the Natural Science Foundation of Heilongjiang Province (JJ2018ZR0081), Harbin Science and Technology Innovation Talent Research Special Funds (2016RAQXJ151), and the Open Project of the State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (no. ES201607).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2018_1310_MOESM1_ESM.pdf (344 kb)
ESM 1 (PDF 343 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, College of ScienceNortheast Forestry UniversityHarbinChina
  2. 2.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina

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