Analytical and Bioanalytical Chemistry

, Volume 411, Issue 3, pp 559–564 | Cite as

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

  • Michaela Tvrdonova
  • Marcela Vlcnovska
  • Lucie Pompeiano Vanickova
  • Viktor Kanicky
  • Vojtech Adam
  • Lena Ascher
  • Norbert Jakubowski
  • Marketa Vaculovicova
  • Tomas VaculovicEmail author
Part of the following topical collections:
  1. Elemental and Molecular Imaging by LA-ICP-MS


In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.


Dot blot Antibody labelling Gold nanoparticles LA-ICP-MS Immunochemistry 



Financial support was provided by Grant Agency of Czech Republic (GACR 17-12774S) and project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Program II. LPV was supported by project 6SA17676 that received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie and which is co-financed by the South Moravian Region under the grant agreement No. 665860.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Michaela Tvrdonova
    • 1
    • 2
  • Marcela Vlcnovska
    • 3
  • Lucie Pompeiano Vanickova
    • 3
    • 4
  • Viktor Kanicky
    • 1
    • 2
  • Vojtech Adam
    • 3
    • 4
  • Lena Ascher
    • 5
  • Norbert Jakubowski
    • 5
  • Marketa Vaculovicova
    • 3
    • 4
  • Tomas Vaculovic
    • 1
    • 4
    Email author
  1. 1.Department of Chemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.CEITECMasaryk UniversityBrnoCzech Republic
  3. 3.Department of Chemistry and BiochemistryMendel University in BrnoBrnoCzech Republic
  4. 4.Central European Institute of TechnologyBrno University of TechnologyBrnoCzech Republic
  5. 5.Bundesanstalt für Materialforschung und -prüfung (BAM)BerlinGermany

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