Analytical and Bioanalytical Chemistry

, Volume 411, Issue 9, pp 1791–1806 | Cite as

Nanoparticles for nucleic-acid-based biosensing: opportunities, challenges, and prospects

  • Susana CampuzanoEmail author
  • Paloma Yáñez-Sedeño
  • José M. PingarrónEmail author
Part of the following topical collections:
  1. Nanoparticles for Bioanalysis


Electrochemical nucleic-acid-based biosensing strategies involving the use of nanoparticles as electrode modifiers and as advanced labels are attractive options for the determination of substances that are relevant clinically, from an environmental perspective, and to food analysis, as these strategies are able to overcome some of the well-known limitations of conventional methodologies for routine applications. In this article, we provide a selective overview of current strategies for nucleic acid electrochemical biosensing based on nanoparticles, in order to demonstrate the relevance and potential of these strategies to readers familiar with this field and to non-experts. The benefits provided by the use of nanoparticles, including enhanced analytical performance of the resulting electrochemical biosensors, as well as the main challenges to be solved and potential future advances in this field are discussed.


Nucleic acids Nanoparticles Electrochemical sensors 



The financial support of the CTQ2015-70023-R and CTQ2015-64402-C2-1-R (Spanish Ministerio de Economía y Competitividad Research Projects) and S2013/MT3029 (NANOAVANSENS Program from the Comunidad de Madrid) are gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

No humans are involved in this study.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departamento de Química Analítica, Facultad de CC. QuímicasUniversidad Complutense de MadridMadridSpain
  2. 2.IMDEA NanoscienceCiudad Universitaria de CantoblancoMadridSpain

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