Methodical studies of the simultaneous determination of anions and cations by IC×CE–MS using arsenic species as model analytes
- 123 Downloads
The separation of the constituents of complex sample mixtures is a challenging task in analytical chemistry. Multidimensional separation systems are widely used to enhance the peak capacity. The comprehensive hyphenation of ion chromatography (IC) and capillary electrophoresis (CE) is promising because the two most important instrumental techniques in ion analysis are combined. In this report a new configuration for capillary anion chromatography is presented enabling the simultaneous IC×CE analysis of anions and cations using a switching valve. Electrospray ionization mass spectrometry (MS) was used for detection. A mixture of organic and inorganic arsenic species served as a model system. The coupling of anion chromatography to CE–MS was done via a modulator enabling periodical injections of the IC effluent into the CE. The injection parameters of the modulator were studied taking into account the complex transport situation.
KeywordsTwo-dimensional separation Capillary electrophoresis Ion chromatography Electrospray ionization Mass spectrometry Arsenic speciation
Compliance with ethical standards
This article does not contain any studies with human participants or animals.
Conflict of interest
The authors declare that they have no conflict of interests.
- 9.Navarro-Reig M, Jaumot J, Baglai A, Vivó-Truyols G, Schoenmakers PJ, Tauler R. Untargeted comprehensive two-dimensional liquid chromatography coupled with high-resolution mass spectrometry analysis of rice metabolome using multivariate curve resolution. Anal Chem. 2017;89(14):7675–83.CrossRefPubMedGoogle Scholar
- 23.Wang T, Ma J, Wu S, Yuan H, Zhang L, Liang Z, et al. Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling. Electrophoresis. 2011;32(20):2848–56.CrossRefPubMedGoogle Scholar