Analytical and Bioanalytical Chemistry

, Volume 410, Issue 21, pp 5165–5172 | Cite as

A molecularly imprinted sensor with enzymatic enhancement of electrochemiluminescence of quantum dots for ultratrace clopyralid determination

  • Qingyu Wang
  • Shuhuai Li
  • Jianping LiEmail author
Research Paper


A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample. The amount of HRP on the molecularly imprinted polymer electrode decreased, and then less H2O2 was catalytically decomposed. Subsequently, the ECL intensity of the CdTe–H2O2 system was enhanced. There was a good linear relationship between ECL intensity and the concentration of CPD in the range from 2.0 × 10-11 to 2.5 × 10-10 mol/L and in the range from 2.5 × 10-10 to 3.5 × 10-8 mol/L. The detection limit was 4.1 × 10-12 mol/L. The sensor was applied to determine CPD in vegetable samples.

Graphical abstract

A molecularly imprinted electrochemiluminescence sensor was fabricated for ultratrace clopyralid determination. The sensitivity was significantly improved with the enhancement of the ECL intensity of quantum dot via the enzymatic reaction of HRP


Quantum dots Molecular imprinting Clopyralid Enzymatic amplification Electrochemiluminescence 



We gratefully acknowledge the financial support from the National Natural Science Foundation of China (nos 21765006 and 21375031), the Natural Science Foundation of Guangxi Province of China (no. 2015GXNSFFA139005), and the High Level Innovation Teams of Guangxi Colleges & Universities and Outstanding Scholars Program (Guijiaoren[2014]49).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethics approval

This work did not involve studies on animals or humans.

Supplementary material

216_2018_1170_MOESM1_ESM.pdf (41 kb)
ESM 1 (PDF 41.3 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry and BioengineeringGuilin University of TechnologyGuilinChina

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