Analytical and Bioanalytical Chemistry

, Volume 410, Issue 20, pp 5085–5092 | Cite as

Electrochemical nonenzymatic sensor for cholesterol determination in food

  • Ksenia DerinaEmail author
  • Elena Korotkova
  • Yekaterina Taishibekova
  • Lyazat Salkeeva
  • Bohumil Kratochvil
  • Jiri Barek
Research Paper


The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier. Stable anodic response was obtained from cholesterol on the modified carbon-based electrode. The sensor has high stability, sensitivity (20 μA mol L−1 cm−2), and a wide linear range from 1 up to 200 μM. The LOD and LOQ values are 1.5 and 5.1 μM, respectively. The developed methods were successfully applied to the above mentioned dairy products.

Graphical abstract


Cholesterol Voltammetry Sensor Smith-Lemli-Opitz syndrome Dairy products 



The research was partly funded from the State program «Science» (project No. 4.5752.2017) and the framework of Tomsk Polytechnic University Competitiveness Enhancement Program grant. JB thanks for financial support of Grant Agency of the Czech Republic (project P206/12/G151).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

No humans are involved in this study.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ksenia Derina
    • 1
    Email author
  • Elena Korotkova
    • 1
  • Yekaterina Taishibekova
    • 2
  • Lyazat Salkeeva
    • 2
  • Bohumil Kratochvil
    • 1
    • 3
  • Jiri Barek
    • 1
    • 4
  1. 1.Physical and Analytical Chemistry Department, Institute of Natural ResourcesTomsk Polytechnic UniversityTomskRussia
  2. 2.Department of Organic Chemistry and Polymers, Faculty of ChemistryKaraganda State UniversityKaragandaKazakhstan
  3. 3.Department of Solid State ChemistryUniversity of Chemistry and TechnologyPrague 6Czech Republic
  4. 4.Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental ElectrochemistryCharles UniversityPrague 2Czech Republic

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