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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 20, pp 4787–4792 | Cite as

Gold-coated polydimethylsiloxane microwells for high-throughput electrochemiluminescence analysis of intracellular glucose at single cells

  • Juan Xia
  • Junyu Zhou
  • Ronggui Zhang
  • Dechen Jiang
  • Depeng Jiang
Communication

Abstract

In this communication, a gold-coated polydimethylsiloxane (PDMS) chip with cell-sized microwells was prepared through a stamping and spraying process that was applied directly for high-throughput electrochemiluminescence (ECL) analysis of intracellular glucose at single cells. As compared with the previous multiple-step fabrication of photoresist-based microwells on the electrode, the preparation process is simple and offers fresh electrode surface for higher luminescence intensity. More luminescence intensity was recorded from cell-retained microwells than that at the planar region among the microwells that was correlated with the content of intracellular glucose. The successful monitoring of intracellular glucose at single cells using this PDMS chip will provide an alternative strategy for high-throughput single-cell analysis.

Graphical abstract

Keywords

Single cell analysis PDMS microwells Electrochemiluminescence imaging Intracellular glucose Cellular heterogeneity 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 81650003) and The Frontiers and Application of Chongqing Science and Technology Commission (No.cstc2015jcyjA10030).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1160_MOESM1_ESM.pdf (320 kb)
ESM 1 (PDF 188 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Juan Xia
    • 1
  • Junyu Zhou
    • 2
  • Ronggui Zhang
    • 3
  • Dechen Jiang
    • 2
  • Depeng Jiang
    • 1
  1. 1.Department of Respiratory Medicine, The Second Affiliated HospitalChongqing Medical UniversityChongqingChina
  2. 2.State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical EngineeringNanjing UniversityNanjingChina
  3. 3.Department of Urinary Surgery, The Second Affiliated HospitalChongqing Medical UniversityChongqingChina

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