Gold-coated polydimethylsiloxane microwells for high-throughput electrochemiluminescence analysis of intracellular glucose at single cells
In this communication, a gold-coated polydimethylsiloxane (PDMS) chip with cell-sized microwells was prepared through a stamping and spraying process that was applied directly for high-throughput electrochemiluminescence (ECL) analysis of intracellular glucose at single cells. As compared with the previous multiple-step fabrication of photoresist-based microwells on the electrode, the preparation process is simple and offers fresh electrode surface for higher luminescence intensity. More luminescence intensity was recorded from cell-retained microwells than that at the planar region among the microwells that was correlated with the content of intracellular glucose. The successful monitoring of intracellular glucose at single cells using this PDMS chip will provide an alternative strategy for high-throughput single-cell analysis.
KeywordsSingle cell analysis PDMS microwells Electrochemiluminescence imaging Intracellular glucose Cellular heterogeneity
This work was supported by the National Natural Science Foundation of China (Nos. 81650003) and The Frontiers and Application of Chongqing Science and Technology Commission (No.cstc2015jcyjA10030).
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Conflict of interest
The authors declare that they have no conflict of interest.