Analytical and Bioanalytical Chemistry

, Volume 410, Issue 1, pp 247–257 | Cite as

Synthesis of multirecognition magnetic molecularly imprinted polymer by atom transfer radical polymerization and its application in magnetic solid-phase extraction

  • Xiang-Jin Kong
  • Chao Zheng
  • Yao-Han Lan
  • Shuai-Shuai Chi
  • Qian Dong
  • Hao-Long Liu
  • Chao Peng
  • Lin-Yi Dong
  • Liang Xu
  • Xian-Hua Wang
Research Paper


In this work, we reported an effective method for the synthesis of a multirecognition magnetic molecularly imprinted polymer (MMIP) with atom transfer radical polymerization (ATRP), using 2,4-diamino-6-methyl-1,3,5-triazine as pseudo-template. The resulting MMIP was characterized in detail by Fourier transform-infrared (FT-IR) spectra, scanning electron microscopy (SEM), thermogravimetic analysis (TGA), and vibrating sample magnetometry (VSM). These results indicated the successful synthesis of MMIP with sufficient thermal stability and magnetic properties. The adsorption experiments were carried out to evaluate the specific selectivity of MMIP related to the spatial structure of target molecules. The MMIP exhibited multirecognition ability and excellent binding capability for melamine (MEL), cyromazine (CYR), triamterene (TAT), diaveridine (DVD), and trimethoprim (TME), and the apparent maximum number of binding sites (Q max) was 77.5, 75.2, 72.5, 69.9, and 70.4 μmol g−1, respectively. The multirecognition MMIP not only possessed adequate magnetic responsiveness for fast separation but also avoided the risk of template leakage on trace component analysis. Therefore, it was suitable for serving as a magnetic solid-phase extraction (MSPE) adsorbent. MSPE coupled with high-performance liquid chromatography analysis was applied to enrich and separate five target molecules from three samples. Recoveries for all target molecules ranged from 81.6 to 91.5% with relative standard deviations of no more than 4.1% (n = 3).

Graphical abstract

Multirecognition property of magnetic molecularly imprinted polymer prepared with pseudo template


Multirecognition Magnetic molecularly imprinted polymer Atom transfer radical polymerization Magnetic solid-phase extraction 



This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 21605114, 21406103), National Student’s Platform for Innovation and Entrepreneurship Training Program (Grant No. 201610062011), China Postdoctoral Science Foundation (Grant No. 2015T80226), and Tianjin Natural Science Foundation (Grant No. 16JCQNJC05500).

Compliance with ethical standards

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Tianjin Medical University and the Ethics Committee of Liaocheng University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2017_716_MOESM1_ESM.pdf (2.6 mb)
ESM 1 (PDF 2.61 MB)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Xiang-Jin Kong
    • 1
  • Chao Zheng
    • 2
  • Yao-Han Lan
    • 2
  • Shuai-Shuai Chi
    • 2
  • Qian Dong
    • 2
  • Hao-Long Liu
    • 2
  • Chao Peng
    • 2
  • Lin-Yi Dong
    • 2
  • Liang Xu
    • 2
    • 3
  • Xian-Hua Wang
    • 2
  1. 1.School of Chemistry and Chemical EngineeringLiaocheng UniversityLiaochengChina
  2. 2.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of PharmacyTianjin Medical UniversityTianjinChina
  3. 3.Tianjin Medical CollegeTianjinChina

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