Analytical and Bioanalytical Chemistry

, Volume 409, Issue 30, pp 7133–7144 | Cite as

A highly selective electrochemical sensor based on molecularly imprinted polypyrrole-modified gold electrode for the determination of glyphosate in cucumber and tap water

  • Chao Zhang
  • Yongxin SheEmail author
  • Tengfei Li
  • Fengnian Zhao
  • Maojun Jin
  • Yirong Guo
  • Lufei Zheng
  • Shanshan Wang
  • Fen Jin
  • Hua Shao
  • Haijin Liu
  • Jing WangEmail author
Research Paper


An electrochemical sensor based on molecularly imprinted polypyrrole (MIPPy) was developed for selective and sensitive detection of the herbicide glyphosate (Gly) in cucumber and tap water samples. The sensor was prepared via synthesis of molecularly imprinted polymers on a gold electrode in the presence of Gly as the template molecule and pyrrole as the functional monomer by cyclic voltammetry (CV). The sensor preparation conditions including the ratio of template to functional monomers, number of CV cycles in the electropolymerization process, the method of template removal, incubation time, and pH were optimized. Under the optimal experimental conditions, the DPV peak currents of hexacyanoferrate/hexacyanoferrite changed linearly with Gly concentration in the range from 5 to 800 ng mL−1, with a detection limit of 0.27 ng mL−1 (S/N = 3). The sensor was used to detect the concentration of Gly in cucumber and tap water samples, with recoveries ranging from 72.70 to 98.96%. The proposed sensor showed excellent selectivity, good stability and reversibility, and could detect the Gly in real samples rapidly and sensitively.

Graphical abstract

Schematic illustration of the experimental procedure to detect Gly using the MIPPy electrode


Electrochemical sensor Molecularly imprinted polypyrrole Glyphosate Cucumber Tap water 



This work was supported by the National Natural Science Foundation of China (Contact No. 31471654), the National Key Technology R&D Program for the 12th five-year plan (2014BAD13B05-05), the Agricultural Public Welfare Project and Innovative Research Team in Chinese academy of agricultural sciences (201203094), and the Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture of China (2016-KF-10).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2017_671_MOESM1_ESM.pdf (219 kb)
ESM 1 (PDF 218 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Chao Zhang
    • 1
  • Yongxin She
    • 1
    Email author
  • Tengfei Li
    • 2
  • Fengnian Zhao
    • 1
  • Maojun Jin
    • 1
  • Yirong Guo
    • 3
  • Lufei Zheng
    • 1
  • Shanshan Wang
    • 1
  • Fen Jin
    • 1
  • Hua Shao
    • 1
  • Haijin Liu
    • 4
  • Jing Wang
    • 1
    Email author
  1. 1.Key Laboratory of Agro-product Quality and Food Safety, Ministry of Agriculture, Institute of Quality Standards & Testing Technology for Agro-ProductsChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Department of Food Science, College of AgricultureHebei University of EngineeringHandanChina
  3. 3.College of Agriculture and Biology TechnologyZhejiang UniversityZhejiangChina
  4. 4.Tibet Testing Center of Quality and Safety for Agricultural and Animal Husbandry ProductsLhasaChina

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