Towards quantification of toxicity of lithium ion battery electrolytes - development and validation of a liquid-liquid extraction GC-MS method for the determination of organic carbonates in cell culture materials
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A novel method based on liquid-liquid extraction with subsequent gas chromatography separation and mass spectrometric detection (GC-MS) for the quantification of organic carbonates in cell culture materials is presented. Method parameters including the choice of extraction solvent, of extraction method and of extraction time were optimised and the method was validated. The setup allowed for determination within a linear range of more than two orders of magnitude. The limits of detection (LODs) were between 0.0002 and 0.002 mmol/L and the repeatability precisions were in the range of 1.5–12.9%. It could be shown that no matrix effects were present and recovery rates between 98 and 104% were achieved. The methodology was applied to cell culture models incubated with commercial lithium ion battery (LIB) electrolytes to gain more insight into the potential toxic effects of these compounds. The stability of the organic carbonates in cell culture medium after incubation was studied. In a porcine model of the blood-cerebrospinal fluid (CSF) barrier, it could be shown that a transfer of organic carbonates into the brain facing compartment took place.
KeywordsLiquid-liquid extraction GC-MS Lithium ion battery (LIB) Organic carbonates Cell culture materials
This work was supported by the German Federal Ministry of Education and Research (BMBF) within the project “SafeBatt” (project grant numbers 03X4631N and 03X4631Q).
Compliance with ethical standards
The primary cultures were obtained from the brains of freshly slaughtered pigs. The brains were collected from a slaughterhouse. The clear advantage is the alternative to the costly, time-consuming and ethically questionable systems derived from experimental animals.
Conflict of interest
The authors have no potential conflict of interest.
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