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Analytical and Bioanalytical Chemistry

, Volume 409, Issue 21, pp 5065–5072 | Cite as

A molecularly imprinted polymer synthesized using β-cyclodextrin as the monomer for the efficient recognition of forchlorfenuron in fruits

  • Yang Cheng
  • Jiyun NieEmail author
  • Zhixia Li
  • Zhen Yan
  • Guofeng Xu
  • Haifei Li
  • Dikai Guan
Research Paper

Abstract

In this study, we have successfully synthesized a forchlorfenuron (CPPU)-imprinted polymer in dimethyl sulfoxide by molecular imprinting technique. In the molecular imprinting, β-cyclodextrin was used as the monomer with 1,6-hexamethylene diisocyanate (HMDI) as the cross-linking agent that formed a complex with forchlorfenuron by inclusion interactions. The adsorption equilibrium was attained in approximately 30 min and the maximum binding capacity was 26.79 mg/g, which indicated that the adsorption kinetics was relatively fast. The results of adsorption and selectivity experiments have shown that the imprinted polymer was able to bind forchlorfenuron specifically and reversibly. The specific recognition of the imprinted polymer for forchlorfenuron resulted from the cooperative effects of inclusion interaction and hydrophobic interaction. This imprinted polymer was also used to accurately determine forchlorfenuron residue in real fruit samples. In the standard spiked strawberry sample, the adsorption recoveries for forchlorfenuron were as high as 90.3, 84.5, and 90.8%, respectively. The binding specificity of the imprinted polymer for forchlorfenuron resulted from the imprinting effect. Therefore, as a reusable material possessing high affinity and selectivity, the imprinted polymer is promising in application to detect pesticide residues in fruit samples. In addition, because β-cyclodextrin is water soluble and can include neutral compounds, this molecularly imprinted polymer can be used to recognize pesticides in aqueous solutions.

Graphical abstract

Schematic demonstration of molecular imprinting and re-binding of forchlorfenuron

Keywords

Molecularly imprinted polymer β-Cyclodextrin Forchlorfenuron Selectivity Inclusion complex 

Notes

Acknowledgements

This work was supported by the National Program for Quality and Safety Risk Assessment of Agricultural Products of China (GJFP2015002, GJFP2016003) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Yang Cheng
    • 1
  • Jiyun Nie
    • 1
    Email author
  • Zhixia Li
    • 1
  • Zhen Yan
    • 1
  • Guofeng Xu
    • 1
  • Haifei Li
    • 1
  • Dikai Guan
    • 1
  1. 1.Institute of Pomology, Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng), Ministry of AgricultureXingchengChina

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