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Analytical and Bioanalytical Chemistry

, Volume 409, Issue 17, pp 4233–4245 | Cite as

Chemical characterisation of the whole plant cell wall of archaeological wood: an integrated approach

  • Luca Zoia
  • Diego TamburiniEmail author
  • Marco OrlandiEmail author
  • Jeannette Jacqueline Łucejko
  • Anika Salanti
  • Eeva-Liisa Tolppa
  • Francesca Modugno
  • Maria Perla Colombini
Research Paper

Abstract

Wood artefacts undergo complex alteration and degradation during ageing, and gaining information on the chemical composition of wood in archaeological artefacts is fundamental to plan conservation strategies. In this work, an integrated analytical approach based on innovative NMR spectroscopy procedures, gel permeation chromatography and analytical pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC-MS) was applied for the first time on archaeological wood from the Oseberg collection (Norway), in order to evaluate the chemical state of preservation of the wood components, without separating them. We adopted ionic liquids (ILs) as non-derivatising solvents, thus obtaining an efficient dissolution of the wood, allowing us to overcome the difficulty of dissolving wood in its native form in conventional molecular solvents. Highly substituted lignocellulosic esters were therefore obtained under mild conditions by reacting the solubilised wood with either acetyl chloride or benzoyl chloride. A phosphytilation reaction was also performed using 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholan. As a result, the functionalised wood developed an enhanced solubility in molecular solvents, thus enabling information about modifications of lignin, depolymerisation of cellulose and structure of lignin-carbohydrate complexes to be obtained by means of spectroscopic (2D-HSQC-NMR and 31P-NMR) and chromatographic (gel permeation chromatography) techniques. Py-GC-MS was used to investigate the degradation undergone by the lignocellulosic components on the basis of their pyrolysis products, without any pre-treatment of the samples. The application of all these combined techniques enabled a comprehensive characterisation of the whole cell wall of archaeological wood and the evaluation of its state of preservation. High depletion of carbohydrates and high extent of lignin oxidation were highlighted in the alum-treated objects, whereas a good preservation state was found for the untreated wood of the Oseberg ship.

Graphical abstract

Keywords

Waterlogged archaeological wood Ionic liquids NMR GPC, Py(HMDS)-GC-MS 

Notes

Acknowledgements

The research was part of the Saving Oseberg project (2014-16) funded by the Norwegian State and the University of Oslo. The authors would like to thank the Museum of Cultural History, University of Oslo for providing the samples of archaeological wood.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Luca Zoia
    • 1
  • Diego Tamburini
    • 2
    • 3
    Email author
  • Marco Orlandi
    • 1
    Email author
  • Jeannette Jacqueline Łucejko
    • 2
    • 4
  • Anika Salanti
    • 1
  • Eeva-Liisa Tolppa
    • 1
  • Francesca Modugno
    • 2
  • Maria Perla Colombini
    • 2
    • 4
  1. 1.Department of Earth and Environmental SciencesUniversity of Milan-BicoccaMilanItaly
  2. 2.Department of Chemistry and Industrial ChemistryUniversity of PisaPisaItaly
  3. 3.Department of Scientific ResearchThe British MuseumLondonUK
  4. 4.National Research CouncilInstitute for the Conservation and Valorization of Cultural Heritage (ICVBC)FlorenceItaly

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