Analytical and Bioanalytical Chemistry

, Volume 408, Issue 29, pp 8559–8567 | Cite as

Highly selective solid-phase extraction sorbents for chloramphenicol determination in food and urine by ion mobility spectrometry

  • Sergio Armenta
  • Miguel de la Guardia
  • Antonio Abad-Fuentes
  • Antonio Abad-Somovilla
  • Francesc A. Esteve-TurrillasEmail author
Research Paper


Different highly selective sorbents have been evaluated for the treatment of food and biological samples to determine chloramphenicol residues by ion mobility spectrometry (IMS). Combination of a selective solid-phase extraction (SPE) and dispersive liquid-liquid microextraction allowed a highly sensitive determination of chloramphenicol in water, milk, honey, and urine samples. The performance of selective SPE supports such as immunoaffinity chromatography (IAC) and molecular imprinted polymers (MIP) have been compared in terms of selectivity, sensitivity, trueness, precision, and reusability. Quantitative recoveries were obtained for chloramphenicol residues, ranging from 91 to 123 % for water, from 99 to 120 % for skimmed milk, and from 95 to 124 % for urine using IAC-IMS and MIP-IMS methods. Quantitative recoveries (from 88 to 104 %) were also achieved for honey samples using IAC-IMS, but low recoveries were obtained using MIP-IMS. The limit of quantification was set at 0.1 μg L−1 which is lower than the minimum required performance limit established by the EU. The proposed methodology is a simple and cost affordable alternative to chromatography methods for the highly sensitive and selective analysis of chloramphenicol residues in food and urine.

Graphical Abstract

Scheme for chloramphenicol determination by selective solid-phase extraction and ion mobility spectrometry


Chloramphenicol Ion mobility spectrometry Immunoaffinity chromatography Molecular imprinted polymer Solid-phase extraction 



Authors gratefully acknowledge the financial support of the Ministerio de Economía y Competitividad (AGL2012-39965-C02-01-02/ALI, CTQ-2012-38635, and CTQ-2014-52841) and Generalitat Valenciana (PROMETEO-II 2014-077).

Compliance with ethical standards

This study has been approved by the appropriate ethics committee and has been performed in accordance with the ethical standards.

Conflict of interest

The authors declare no competing financial or nonfinancial interest.

Supplementary material

216_2016_9995_MOESM1_ESM.pdf (347 kb)
ESM 1 (PDF 346 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Sergio Armenta
    • 1
  • Miguel de la Guardia
    • 1
  • Antonio Abad-Fuentes
    • 2
  • Antonio Abad-Somovilla
    • 3
  • Francesc A. Esteve-Turrillas
    • 1
    Email author
  1. 1.Department of Analytical ChemistryUniversity of ValenciaBurjassotSpain
  2. 2.Department of Biotechnology, Institute of Agrochemistry and Food TechnologyConsejo Superior de Investigaciones Científicas (IATA-CSIC)PaternaSpain
  3. 3.Department of Organic ChemistryUniversity of ValenciaBurjassotSpain

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