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Analytical and Bioanalytical Chemistry

, Volume 408, Issue 29, pp 8257–8262 | Cite as

Estrogen receptor preparation effects on the receptor–DNA interaction by surface plasmon resonance

  • Sandrine Bayle
  • Joel Chopineau
  • Benoit Roig
  • Denis HabauzitEmail author
Feature Article

Abstract

Up to now, several studies have investigated estrogen receptor (ER)–estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER–ERE interactions.

Keywords

Surface plasmon resonance Estrogen Estrogen receptor Dimerization Temperature 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Sandrine Bayle
    • 1
  • Joel Chopineau
    • 2
    • 3
  • Benoit Roig
    • 2
    • 4
  • Denis Habauzit
    • 1
    • 5
    • 6
    Email author
  1. 1.Laboratoire Génie de l’Environnement IndustrielEcole des Mines d’AlèsAlès CedexFrance
  2. 2.Université de NîmesNimesFrance
  3. 3.Institut Charles Gerhardt MontpellierUMR 5253 CNRS-ENSCM-UM Ecole Nationale Supérieure de ChimieMontpellierFrance
  4. 4.EA7352 CHROMEUniversité de NîmesNimesFrance
  5. 5.Université de Rennes 1RennesFrance
  6. 6.Institut National de la Santé et de la Recherche Médicale (Inserm), Institut de Recherche en Santé, Environnement et Travail (Irset – Inserm U1085)RennesFrance

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