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Miniaturized analytical instrumentation for electrochemiluminescence assays: a spectrometer and a photodiode-based device

Abstract

Herein, a new miniaturized analytical instrumentation for electrochemiluminescence (ECL) assays is presented. A photodiode integrated in an ECL cell combined with a potentiostat/galvanostat, all integrated in a one-piece instrument (μSTAT ECL), was developed. In addition, a complementary micro-spectrometer integrated in a similar ECL cell for luminescence spectra recording is also proposed. Both cells are intended to be used with screen-printed electrodes and all the devices are portable and small sized. Their performance was corroborated with two innovative proofs-of-concept that centered on the luminol transduction chemistry: a first time reported ECL assay based on the enzymatic reaction between an indoxyl substrate and the enzyme alkaline phosphatase, and the electrochemiluminescence resonance energy transfer (ECL-RET) process triggered by the electro-oxidized luminol to the acceptor fluorescein. The photodiode system revealed to be more sensitive than the spectrometer device in collecting the light; however, with the latter, it is possible to discriminate different luminescent species according to their maximum wavelength emission, which is extremely useful for carrying out simple and simultaneous ECL multiplex analyzes. The spectrometer device works as an excellent accessory to couple with the μSTAT ECL instrument, complementing the experiments.

Schematic representation of the ECL-RET: from luminol–H2O2 system to fluorescein, the micro-spectrometer for the light collection and the 3D representation of the ECL-RET reaction.

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References

  1. 1.

    Fähnrich KA, Pravda M, Guilbault GG. Talanta. 2001;54:531–59.

  2. 2.

    Richter MM. Chem Rev. 2004;104:3003–36.

  3. 3.

    Hu L, Guobao X. Chem Soc Rev. 2010;39:3275–304.

  4. 4.

    Ballesta Claver J, Valencia Mirón MC, Capitán-Vallvey LF. Analyst (Cambridge, U K). 2009;134:1423–32.

  5. 5.

    Deng S, Ju H. Analyst (Cambridge, U K). 2013;138:43–61.

  6. 6.

    Muzyka K. Biosens Bioelectron. 2014;54:393–407.

  7. 7.

    Kirschbaum SEK, Baeumner AJ. Anal Bioanal Chem. 2015;407:3911–26.

  8. 8.

    Spehar-Délèze AM, Gransee R, Martinez-Montequin S, Bejarano-Nosas D, Dulay S, Julich S, et al. Anal Bioanal Chem. 2015;407:6657–67.

  9. 9.

    Chi Y, Duan J, Lin S, Chen G. Anal Chem. 2006;78:1568–73.

  10. 10.

    Sun Y, Zhang Z, Xi Z. Anal Chim Acta. 2008;623:96–100.

  11. 11.

    Wang J, Yang Z, Wang X, Yang N. Talanta. 2008;76:85–90.

  12. 12.

    Bertoncello P, Stewart AJ, Dennany L. Anal Bioanal Chem. 2014;406:5573–87.

  13. 13.

    Carvajal MA, Ballesta-Claver J, Morales DP, Palma AJ, Valencia-Mirón MC, Capitán-Vallvey LF. Sensors Actuators B Chem. 2012;169:46–53.

  14. 14.

    Capitán-Vallvey LF, Palma AJ. Anal Chim Acta. 2011;696:27–46.

  15. 15.

    Carvajal MA, Ballesta-Claver J, Martínez-Olmos A, Capitán-Vallvey LF, Palma AJ. Sensors Actuators B Chem. 2015;221:956–61.

  16. 16.

    Doeven EH, Barbante GJ, Hogan CF, Francis PS. ChemPlusChem. 2015;80:456–70.

  17. 17.

    Price CP, Newman DJ. Stockton Press. New: York; 1991.

  18. 18.

    Niwa O, Xu Y, Halsall HB, Heineman WR. Anal Chem. 1993;65:1559–63.

  19. 19.

    Fernández-Sánchez C, Costa-García A. Biosens Bioelectron. 1997;12:403–13.

  20. 20.

    Wilson MS, Rauth RD. Biosens Bioelectron. 2004;19:693–9.

  21. 21.

    Fernández-Sánchez C, Costa-García A. Electroanalysis. 1998;10:249–55.

  22. 22.

    Fanjul-Bolado P, González-García MB, Costa-García A. Talanta. 2004;64:452–7.

  23. 23.

    Fanjul-Bolado P, Hernández-Santos D, González-García MB, Costa-García A. Anal Chem. 2007;79:5272–7.

  24. 24.

    Neves MMPS, González-García MB, Nouw HPA, Costa-García A. Biosens Bioelectron. 2012;31:95–100.

  25. 25.

    Neves MMPS, González-García MB, Hernández-Santos D, Fanjul-Bolado P. Talanta. 2015;144:283–8.

  26. 26.

    Ghosh SS, Gehr TW, Ghosh S. Molecules. 2014;19:20139–56.

  27. 27.

    Torres PU, Bover J, Mazzaferro S, de Vernejoul MC, Cohen-Solal M. Semin Nephrol. 2014;34:612–25.

  28. 28.

    Jadon DR, Nightingale AL, McHugh NJ, Lindsay MA, Korendowych E, Sengupta R. J Rheumatol. 2015;42:21–30.

  29. 29.

    Mahant VK Eur. Patent WOWO 96/04400 (1996).

  30. 30.

    Mahant VK. Biotech Adv. 1997;15:484.

  31. 31.

    Navas Díaz A, González García JA, Lovillo J. J Biolumin Chemilumin. 1997;12:199–205.

  32. 32.

    Mazzei F, Botrè F, Montilla S, Pilloton R, Podestà E, Botrè C. J Electroanal Chem. 2004;574:95–100.

  33. 33.

    Nichols JJ, King-Smith PE, Hinel EA, Thangavelu M, Nichols KK. Invest Ophthalmol Vis Sci. 2012;53:5426–32.

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Author information

Correspondence to Pablo Fanjul-Bolado.

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The authors declare that they have no conflict of interest.

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Published in the topical collection Analytical Electrochemiluminescence with guest editors Hua Cui, Francesco Paolucci, Neso Sojic, and Guobao Xu.

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Neves, M.M.P.S., Bobes-Limenes, P., Pérez-Junquera, A. et al. Miniaturized analytical instrumentation for electrochemiluminescence assays: a spectrometer and a photodiode-based device. Anal Bioanal Chem 408, 7121–7127 (2016) doi:10.1007/s00216-016-9669-7

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Keywords

  • Electrochemiluminescence
  • Portable instrumentation
  • Screen-printed electrode
  • Miniaturized photodiode and spectrometer
  • Luminol
  • Alkaline phosphatase