Analytical and Bioanalytical Chemistry

, Volume 408, Issue 20, pp 5527–5535 | Cite as

Experimental and computational studies on molecularly imprinted solid-phase extraction for gonyautoxins 2,3 from dinoflagellate Alexandrium minutum

  • Ziru Lian
  • Hai-Bei LiEmail author
  • Jiangtao WangEmail author
Research Paper


An innovative and effective extraction procedure based on molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation of gonyautoxins 2,3 (GTX2,3) from Alexandrium minutum sample. Molecularly imprinted polymer microspheres were prepared by suspension polymerization and and were employed as sorbents for the solid-phase extraction of GTX2,3. An off-line MISPE protocol was optimized. Subsequently, the extract samples from A. minutum were analyzed. The results showed that the interference matrices in the extract were obviously cleaned up by MISPE procedures. This outcome enabled the direct extraction of GTX2,3 in A. minutum samples with extraction efficiency as high as 83 %, rather significantly, without any need for a cleanup step prior to the extraction. Furthermore, computational approach also provided direct evidences of the high selective isolation of GTX2,3 from the microalgal extracts.


Gonyautoxins 2,3 Molecularly imprinted solid-phase extraction Alexandrium minutum Computational approach 



This project was supported by the National Natural Science Foundation of China (No.41506092), the Public Science and Technology Research Funds Projects of Ocean (No. 201505034), the Natural Science Foundation of Shandong Province of China (No. ZR2014BQ015), and the China Postdoctoral Science Foundation (No. 2014M551892).

Compliance with ethical standard

Conflict of interest

The authors declared that they have no conflict of interest.

Supplementary material

216_2016_9644_MOESM1_ESM.pdf (86 kb)
ESM. 1 (PDF 86.3 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Marine CollegeShandong UniversityWeihaiChina
  2. 2.Key Laboratory of Marine Chemistry Theory and Technology, Ministry of EducationOcean University of ChinaQingdaoChina

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