Analytical and Bioanalytical Chemistry

, Volume 408, Issue 16, pp 4287–4295 | Cite as

Electrochemical sensor based on molecularly imprinted polymer for sensitive and selective determination of metronidazole via two different approaches

  • Jie Liu
  • Hui Tang
  • Bo Zhang
  • Xiling Deng
  • Feilang Zhao
  • Peng Zuo
  • Bang-Ce Ye
  • Yingchun LiEmail author
Research Paper


A molecularly imprinted polymer decorated glassy carbon electrode (MIP/GCE) is facilely developed into an electrochemical sensing platform for detection of metronidazole (MNZ). MIP preparation was carried out via in situ electropolymerization and o-phenylenediamine was selected as the optimal functional monomer. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to characterize and assess the performance of the so-obtained sensor. In particular, two assay methods, which are based on different principles, were involved in the detection procedure. One is based on MIP/catalysis (Method І) and the other is MIP/gate effect (Method II). Comparison of these two methods was made in the aspects including detection range, sensitivity, accuracy, selectivity, repeatability, and long-term stability. It is found that Method І affords a lower detection limit of 3.33 × 10−10 M (S/N = 3) while the detection limit of Method II is 6.67 × 10−10 M (S/N = 3). The linear range of Method І and II is 1.0 × 10−9 to 1.0 × 10−8 M and 2.0 × 10−9 to 1.0 × 10−7 M, respectively. The MIP/GCE exhibits good recognition ability towards the template molecule-MNZ in the presence of the analogues of MNZ and other interferents, which can be ascribed to the successful imprinting effect during MIP membrane preparation.

Graphical Abstract

Procedure for fabricating MIP/GCE and its application in detecting metronidazole in serum


Electrochemical sensor Electro-catalysis Gate effect Metronidazole Molecularly imprinted polymer 



This project was financially supported by the Major Program for Science and Technology Development of Shihezi University (gxjs2014-zdgg04), National Natural Science Foundation of China (81260487, 81460543, 21575089), the Scientific Research Foundation for the Returned Overseas Chinese Scholars from Ministry of Human Resources and Social Security of China (RSLX201301), the Pairing Program of Shihezi University with Eminent Scholar in Elite University (SDJDZ201502, SDJDZ201503), and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

Compliance with ethical standards

Conflict of interest

The authors declare no Conflict of interest.

Supplementary material

216_2016_9520_MOESM1_ESM.pdf (342 kb)
ESM 1 (PDF 342 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jie Liu
    • 1
  • Hui Tang
    • 1
  • Bo Zhang
    • 1
  • Xiling Deng
    • 1
  • Feilang Zhao
    • 3
  • Peng Zuo
    • 4
  • Bang-Ce Ye
    • 2
    • 4
  • Yingchun Li
    • 1
    • 2
    • 3
    Email author
  1. 1.Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of PharmacyShihezi UniversityShiheziChina
  2. 2.Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical EngineeringShihezi UniversityShiheziChina
  3. 3.Jiangsu Devote Instrumental Science and Technology Co., Ltd.Huai’anChina
  4. 4.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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