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Analytical and Bioanalytical Chemistry

, Volume 408, Issue 14, pp 3751–3759 | Cite as

A novel four-dimensional analytical approach for analysis of complex samples

  • Susanne Stephan
  • Cornelia Jakob
  • Jörg Hippler
  • Oliver J. SchmitzEmail author
Research Paper

Abstract

A two-dimensional LC (2D-LC) method, based on the work of Erni and Frei in 1978, was developed and coupled to an ion mobility-high-resolution mass spectrometer (IM-MS), which enabled the separation of complex samples in four dimensions (2D-LC, ion mobility spectrometry (IMS), and mass spectrometry (MS)). This approach works as a continuous multiheart-cutting LC system, using a long modulation time of 4 min, which allows the complete transfer of most of the first - dimension peaks to the second - dimension column without fractionation, in comparison to comprehensive two-dimensional liquid chromatography. Hence, each compound delivers only one peak in the second dimension, which simplifies the data handling even when ion mobility spectrometry as a third and mass spectrometry as a fourth dimension are introduced. The analysis of a plant extract from Ginkgo biloba shows the separation power of this four-dimensional separation method with a calculated total peak capacity of more than 8700. Furthermore, the advantage of ion mobility for characterizing unknown compounds by their collision cross section (CCS) and accurate mass in a non-target approach is shown for different matrices like plant extracts and coffee.

Graphical abstract

Principle of the four-dimensional separation

Keywords

2D-LC CCS Ginkgo biloba IM-qTOF-MS Ion mobility LC+LC 

Notes

Acknowledgments

We are thankful to Agilent for the third Infinity pump system and Phenomenex for the HPLC columns.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2016_9460_MOESM1_ESM.pdf (531 kb)
ESM 1 (PDF 530 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Susanne Stephan
    • 1
  • Cornelia Jakob
    • 1
  • Jörg Hippler
    • 1
  • Oliver J. Schmitz
    • 1
    Email author
  1. 1.Applied Analytical ChemistryUniversity of Duisburg-EssenEssenGermany

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