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Analytical and Bioanalytical Chemistry

, Volume 409, Issue 9, pp 2285–2295 | Cite as

Assessment of human telomeric G-quadruplex structures using surface-enhanced Raman spectroscopy

  • Snežana MiljanićEmail author
  • Marina Ratkaj
  • Marija Matković
  • Ivo Piantanida
  • Paola Gratteri
  • Carla Bazzicalupi
Research Paper

Abstract

G-Quadruplex (G4) structures of a human telomeric 24-mer (5′-TTAGGGTTAGGGTTAGGGTTAGGG-3′) sequence (Tel24) stabilized by sodium and potassium ions have been assessed using surface-enhanced Raman scattering (SERS) spectroscopy. The distinctive SERS spectra of Tel24 in the presence of 100 mM Na+ and 100 mM K+ were obtained and the SERS bands characteristic of the antiparallel basket-type and the mixed hybrid (3+1) structures, respectively, were identified and assigned. The influence of the SERS - active substrate on the scattering enhancement was studied using citrate- and chloride-covered silver nanoparticles, in the absence and presence of the aggregating agent (0.1 M Na2SO4 and 0.1 M K2SO4). The highly reproducible SERS spectra of Tel24 obtained in various SERS active media indicated the same adsorption mechanism of the cation - stabilized G-quadruplexes onto the metal surface, regardless of the silver colloid. The remarkable resemblance between the circular dichroism (CD) spectra of the Tel24 structures with and without the colloid confirmed that interaction with the enhancing silver surface did not affect the stability of the formed G4 structures. The presented study pointed to a great potential of the SERS spectroscopy for the sensitive structural analysis of various G4 topologies.

Graphical Abstract

SERS spectroscopy allowed identification of Na+ stabilized antiparallel basket-type and K+ stabilized hybrid (3+1) structures of the same 24-mer human telomeric sequence

Keywords

Surface-enhanced Raman spectroscopy G-Quadruplex Human telomere Silver colloid DNA structure 

Notes

Acknowledgements

This work was supported by the Ministry of Science, Education and Sports of the Republic of Croatia and the Croatian Science Foundation (grant number 1477).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2016_172_MOESM1_ESM.pdf (154 kb)
ESM 1 (PDF 153 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Snežana Miljanić
    • 1
    Email author
  • Marina Ratkaj
    • 2
  • Marija Matković
    • 3
  • Ivo Piantanida
    • 3
  • Paola Gratteri
    • 4
  • Carla Bazzicalupi
    • 5
  1. 1.Division of Analytical Chemistry, Department of Chemistry, Faculty of ScienceUniversity of ZagrebZagrebCroatia
  2. 2.Teva Pharmaceutical Industries Ltd., Research and DevelopmentPLIVA CroatiaZagrebCroatia
  3. 3.Division of Organic Chemistry and BiochemistryRuđer Bošković InstituteZagrebCroatia
  4. 4.Department NEUROFARBA—Pharmaceutical and nutraceutical section, Laboratory of Molecular Modeling Cheminformatics & QSARUniversity of FirenzeSesto FiorentinoItaly
  5. 5.Department of Chemistry “Ugo Schiff”University of FirenzeSesto FiorentinoItaly

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