Analytical and Bioanalytical Chemistry

, Volume 409, Issue 7, pp 1827–1836 | Cite as

SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins

  • Ulrike Anders
  • Jonas V. Schaefer
  • Fatima-Ezzahra Hibti
  • Chiraz Frydman
  • Detlev Suckau
  • Andreas Plückthun
  • Renato Zenobi
Research Paper

Abstract

We report on the direct coupling of surface plasmon resonance imaging (SPRi) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for the investigation of specific, non-covalent interactions, using the example of designed ankyrin repeat proteins (DARPins) and ribosomal protein S6 kinase 2 (RPS6KA2) directly from lysate of SH-SY5Y cells, derived from human bone marrow. Due to an array format, tracing of binding kinetics of numerous DARPins simultaneously and in real time becomes possible. By optimizing both the proteolytic digest directly on the SPRi chip (amount of trypsin, incubation time, and temperature) as well as the MALDI matrix application (concentration of matrix and number of spray cycles), we are able to identify the specific interaction with RPS6KA2 directly from the cell lysate at a surface coverage of only 0.8 fmol/mm2.

Graphical Abstract

Workflow of the direct coupling of SPRi with MALDI mass spectrometry

Keywords

Surface plasmon resonance imaging (SPRi) MALDI mass spectrometry Designed ankyrin repeat proteins (DAPRins) Cell lysate Label-free 

Notes

Acknowledgements

We thank Horiba Jobin Yvon for the loan of an SPRi instrument and Bruker Daltonics and Horiba Jobin Yvon for jointly funding 1.5 years of a Ph.D. studentship awarded to U.A. We also thank all current and former members of the High-Throughput Binder Selection Facility for their contribution to the establishment of the semi-automated ribosome display that resulted in the used DARPin binders. The RPS6KA2 target used for DARPin selection and the experiments described here was kindly provided by Dr. Susanne Müller-Knapp and Tracy Keates (SGC Oxford). Chia-Lung Yang (group of Prof. Olga Shakhova) kindly provided the cell lysate of SH-SY5Y (Department of Oncology, University Hospital Zurich).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2016_127_MOESM1_ESM.pdf (3.2 mb)
ESM 1(PDF 3.18 MB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ulrike Anders
    • 1
  • Jonas V. Schaefer
    • 2
  • Fatima-Ezzahra Hibti
    • 3
  • Chiraz Frydman
    • 3
  • Detlev Suckau
    • 4
  • Andreas Plückthun
    • 2
  • Renato Zenobi
    • 1
  1. 1.Department of Chemistry and Applied BiosciencesETH ZurichZurichSwitzerland
  2. 2.Department of BiochemistryUniversity of ZurichZurichSwitzerland
  3. 3.Horiba Jobin Yvon S.A.S.PalaiseauFrance
  4. 4.Bruker DaltonicsBremenGermany

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