A plasmonic ELISA for the naked-eye detection of chromium ions in water samples
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Here, we describe the development of a triangular silver nanoprism (AgNPR) etching-based plasmonic ELISA for the colorimetric determination of Cr(III) levels in environmental water samples. This involved the creation of a novel signal generation system (substrate reaction solution) for a competitive ELISA in which hydrogen peroxide (H2O2) is used to etch triangular AgNPRs, inducing a change in color. This is achieved by controlling the H2O2 concentration that remains after degradation by catalase, which is conjugated to the secondary antibody of the ELISA. Because the degree of color change and the shift in the absorption spectrum of the substrate reaction solution are closely correlated with the Cr(III) concentration, this plasmonic ELISA can be used not only for the quantification of Cr(III) concentrations ranging from 3.13 to 50 ng/mL, with a limit of detection (LOD) of 3.13 ng/mL, but also for the visual detection (indicated by a color change from blue to mauve) of Cr(III) with a sensitivity of 6.25 ng/mL by the naked eye. Therefore, the plasmonic ELISA developed in this work represents a new strategy for heavy metal ion detection and has high potential applicability in resource-constrained areas.
KeywordsTriangular silver nanoprism Colorimetric Plasmonic ELISA Chromium ion
This work was supported by the National Key Research and Development Program of China (2016YFD0500600), the Technology Research Program of Guangzhou City (201508020100), the Technology Research Program of Guangdong Province (2013B010404027), and the Guangdong Innovative and Entrepreneurial Research Team Program (201301S0105240297). This manuscript was edited and proof-read by NPG Language Editing.
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Conflict of interest
The authors declare that they have no conflict of interest.
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