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Analytical and Bioanalytical Chemistry

, Volume 408, Issue 7, pp 1909–1916 | Cite as

High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry

  • Veerendra Kalyan Jagannadh
  • Bindu Prabhath Bhat
  • Lourdes Albina Nirupa Julius
  • Sai Siva GorthiEmail author
Research Paper

Abstract

In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer.

Keywords

High throughput screening Clinical / biomedical analysis Microfluidic biosensors 

Notes

Acknowledgments

The authors thank Mr. Abhishek Pathak for preparing blood samples for these experiments. The authors acknowledge funding from Biotechnology Ignition Grant (BIG) of BIRAC. BIG team also acknowledges the support of incubation center, Robert-Bosch Center for Cyber-Physical Systems (RBCCPS), IISc.

Compliance with ethical standards

Conflicts of interests

The authors declare no conflicts of Interest.

Supplementary material

216_2015_9301_MOESM1_ESM.pdf (4.6 mb)
(PDF 8.72 MB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Veerendra Kalyan Jagannadh
    • 1
  • Bindu Prabhath Bhat
    • 2
  • Lourdes Albina Nirupa Julius
    • 2
  • Sai Siva Gorthi
    • 1
    • 2
    Email author
  1. 1.Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied PhysicsIndian Institute of Science (IISc)BangaloreIndia
  2. 2.Robert Bosch Centre For Cyber-Physical SystemsIndian Institute of Science (IISc)BangaloreIndia

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