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Analytical and Bioanalytical Chemistry

, Volume 408, Issue 7, pp 1871–1878 | Cite as

Analysis of saccharides in beverages by HPLC with direct UV detection

  • Thomas Schmid
  • Benedikt Baumann
  • Markus Himmelsbach
  • Christian W. Klampfl
  • Wolfgang Buchberger
Research Paper

Abstract

The present study demonstrates the suitability of direct UV detection for saccharide analysis in HPLC. Under highly alkaline conditions, the non-UV absorbing saccharides are converted by a photo-initiated chemical reaction in the detection cell into malonenolate, which can be detected at 266 nm. A straightforward method for such direct UV detection of saccharides after their separation by anion-exchange chromatography was developed and successfully applied to several beverage samples. Investigation and optimization of the influencing factors using design of experiment resulted in a baseline separation of glucose, fructose, and sucrose within 6 min and LOD values below 0.2 mg L−1. In addition, a fast, simple and cost-effective flow injection method was developed to estimate the total saccharide concentration. The results of this method applied to beverage samples are in good agreement with the chromatographic method as well as to the saccharide concentration stated by the manufacturer. Finally, a comparison of different commercially available UV detectors and detector cells revealed that sensitive detection requires the use of recently introduced flow cells with extended path length.

Graphical Abstract

Online direct UV detection of saccharides in HPLC, which is possible due to a photo-initiated conversion of the saccharides into malonenolate

Keywords

Anion-exchange chromatography Direct UV detection HPLC Saccharide 

Notes

Acknowledgments

The authors thank Andreas Kitzler from Agilent Technologies for technical support regarding HPLC instrumentation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Thomas Schmid
    • 1
  • Benedikt Baumann
    • 1
  • Markus Himmelsbach
    • 1
  • Christian W. Klampfl
    • 1
  • Wolfgang Buchberger
    • 1
  1. 1.Institute of Analytical ChemistryJohannes Kepler University LinzLinzAustria

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