Analytical and Bioanalytical Chemistry

, Volume 408, Issue 10, pp 2407–2424 | Cite as

Recent advances in the determination of tocopherols in biological fluids: from sample pretreatment and liquid chromatography to clinical studies

  • Barbora Cervinkova
  • Lenka Kujovska Krcmova
  • Dagmar Solichova
  • Bohuslav Melichar
  • Petr Solich
Review

Abstract

Vitamin E comprises eight related compounds: α-, β-, γ-, δ-tocopherols and α-, β-, γ-, δ-tocotrienols. In the past, α-tocopherol has been the isomer that was studied most, and its anti-inflammatory and anti-proliferative effects have been described. Therefore, many prevention trials have investigated the effect of α-tocopherol on human health. Current research studies have also defined the important roles of other tocopherols, such as anti-inflammatory, anti-proliferative and cancer preventative effects. Knowledge of the individual tocopherols could help to understand their roles in various metabolic pathways. This review summarizes the recent trends in sample pretreatment, liquid chromatography and selected applications of the determination of tocopherols in various biological materials. The relationship between tocopherol isomers and serious diseases is also described.

Graphical Abstract

Article structure

Keywords

Tocopherols Antioxidants Clinical trials Biological samples Sample pretreatment Liquid chromatography 

Notes

Acknowledgments

This work was supported by projects Specific University Research (SVV) 260 184, the European Social Fund and the state budget of the Czech Republic, TEAB, project no. CZ.1.07/2.3.00/20.0235, Internal Grant Agency Ministry of Health Czech Republic (IGA MH CR) NT 14265-3/2013, IGA MH CR NT 13564-4/2012, IGA MH CR NT 13566-4/2012 and MH CZ - DRO (University Hospital Hradec Kralove (UHHK), 00179906).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Barbora Cervinkova
    • 1
    • 2
  • Lenka Kujovska Krcmova
    • 1
    • 2
  • Dagmar Solichova
    • 2
  • Bohuslav Melichar
    • 3
  • Petr Solich
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of PharmacyCharles University in PragueHradec KraloveCzech Republic
  2. 2.3rd Internal Gerontometabolic ClinicUniversity HospitalHradec KraloveCzech Republic
  3. 3.Department of Oncology, Faculty of Medicine and Dentistry and University HospitalPalacky UniversityOlomoucCzech Republic

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