Analytical and Bioanalytical Chemistry

, Volume 408, Issue 2, pp 609–618 | Cite as

A nanoaggregate-on-mirror platform for molecular and biomolecular detection by surface-enhanced Raman spectroscopy

  • Gregory Q. Wallace
  • Mohammadali Tabatabaei
  • Mariachiara S. Zuin
  • Mark S. Workentin
  • François Lagugné-Labarthet
Research Paper

Abstract

A nanoaggregate-on-mirror (NAOM) structure has been developed for molecular and biomolecular detection using surface-enhanced Raman spectroscopy (SERS). The smooth surface of the gold mirror allows for simple and homogeneous functionalization, while the introduction of the nanoaggregates enhances the Raman signal of the molecule(s) in the vicinity of the aggregate-mirror junction. This is evidenced by functionalizing the gold mirror with 4-nitrothiophenol, and the further addition of gold nanoaggregates promotes local SERS activity only in the areas with the nanoaggregates. The application of the NAOM platform for biomolecular detection is highlighted using glucose and H2O2 as molecules of interest. In both cases, the gold mirror is functionalized with 4-mercaptophenylboronic acid (4-MPBA). Upon exposure to glucose, the boronic acid moiety of 4-MPBA forms a cyclic boronate ester. Once the nanoaggregates are added to the surface, detection of glucose is possible without the use of an enzyme. This method of indirect detection provides a limit of detection of 0.05 mM, along with a linear range of detection from 0.1 to 15 mM for glucose, encompassing the physiological range of blood glucose concentration. The detection of H2O2 is achieved with optical inspection and SERS. The H2O2 interferes with the coating of the gold mirror, enabling qualitative detection by visual inspection. Simultaneously, the H2O2 reacts with the boronic acid to form a phenol, a change that is detected by SERS.

Graphical abstract

A nanoaggregate-on-mirror platform is SERS-active over the aggregate regions. When functionalized with a Raman reported the platform can be used to detect glucose based on changes to the observed SERS signal

Keywords

Surface-enhanced Raman spectroscopy Nanoaggregate-on-mirror 4-Mercaptophenylboronic acid Glucose Hydrogen peroxide 

Notes

Acknowledgments

The authors gratefully acknowledge the Nanofabrication Facility at Western University. This research was funded by the Canada Research Chairs program in “Photonics and Nanosciences” (F.L.-L.). The authors also acknowledge the Natural Sciences and Engineering Research Council of Canada.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2015_9142_MOESM1_ESM.pdf (100 kb)
ESM 1(PDF 100 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Gregory Q. Wallace
    • 1
    • 2
  • Mohammadali Tabatabaei
    • 1
    • 2
  • Mariachiara S. Zuin
    • 1
    • 2
  • Mark S. Workentin
    • 1
    • 2
  • François Lagugné-Labarthet
    • 1
    • 2
  1. 1.Department of ChemistryUniversity of Western OntarioLondonCanada
  2. 2.Centre for Advanced Materials and Biomaterials ResearchUniversity of Western OntarioLondonCanada

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