Multiresidue method for the quantitation of 20 pesticides in aquatic products
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As the consumption of aquatic products increased, the need for regulation of pesticide residues in aquatic products also emerged. Thus, in this study, a scheduled multiple reaction monitoring (sMRM) method employing a novel extraction and purification step based on QuEChERS with EDTA was developed for the simultaneous quantitation of 20 pesticides (alachlor, aldicarb, carbofuran, diazinon, dimethoate, dimethomorph, ethoprophos, ferimzone, fluridone, hexaconazole, iprobenfos, malathion, methidathion, methiocarb, phenthoate, phosalone, phosmet, phosphamidon, pirimicarb, and simazine) in aquatic products. Additionally, the present method was validated in the aspects of specificity, linearity (r ≥ 0.980), sensitivity (the limit of quantitation (LOQ) ≤ 5 ng/g), relative standard deviation, RSD (1.0 % ≤ RSD ≤ 19.4 %), and recovery (60.1 % ≤ recovery ≤ 117.9 %). Finally, the validated method was applied for the determination of the 20 pesticide residues in eel and shrimp purchased from local food markets. In the present study, QuEChERS with EDTA was successfully expanded to residual pesticide analysis for the first time. The present method could contribute to the rapid and successful establishment of the positive list system in South Korea.
KeywordsMultiresidue Pesticides Aquatic products QuEChERS LC-MS/MS
We gratefully thank Dr. Tae-Hyun Kim (Soonchunhyang Univeristy) for technical advice. This research was supported by a grant (14162MFDS854) from Ministry of Food and Drug Safety, Republic of Korea in 2014.
Conflict of interest
The authors declare that they have no conflict of interest.
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