Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement
Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a method based on UHPLC-MS/MS measurement and dedicated to 13 estrogenic EDCs was developed and applied to biological matrices. Two molecular recognition-based strategies, either molecular imprinted polymer (MIP) with phenolic template or estrogen receptors (ERα) immobilized on a sorbent, were assessed in terms of recovery and purification efficiency. Both approaches demonstrated their suitability to measure ultra-trace levels of estrogenic EDCs in aqueous samples. Applicability of the MIP procedure to urine and serum samples has also been demonstrated.
KeywordsEstrogenic endocrine disrupting chemicals Molecular imprinted polymer Estrogen receptors Biological matrices UHPLC-MS/MS
The authors want to express their special thanks to all the volunteers who have permitted the collection of biological samples exploited in this work. They also thank the Centre Hospitalier Universitaire de Toulouse as the promoter of the corresponding clinical protocol.
Conflict of Interest
The authors declare no conflict of interest.
- 3.Shen Y, Ren M-L, Feng X, Gao Y-X, Xu Q, Cai Y-L (2014) Eur J Obstet Gynecol Reprod Biol 178:137–137Google Scholar
- 20.Nguyen HP, Li L, Gatson JW, Maass D, Wigginton JG, Simpkins JW, Schug KA (2011) J Pharma Biomed Anal 54(4):830–837Google Scholar
- 22.Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (Text with EEA relevance) (notified under document number C(2002) 3044). Off J Eur Commun 2002:L221, 8Google Scholar
- 31.Delfosse V, Grimaldi M, Cavaillès V, Balaguer P, Bourguet W (2014) Environ Health Perspect 122(12):1306–1313Google Scholar