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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 29, pp 8713–8723 | Cite as

Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement

  • Radia Bousoumah
  • Jean Philippe AntignacEmail author
  • Valérie Camel
  • Marina Grimaldi
  • Patrick Balaguer
  • Frederique Courant
  • Emmanuelle Bichon
  • Marie-Line Morvan
  • Bruno Le Bizec
Research Paper

Abstract

Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a method based on UHPLC-MS/MS measurement and dedicated to 13 estrogenic EDCs was developed and applied to biological matrices. Two molecular recognition-based strategies, either molecular imprinted polymer (MIP) with phenolic template or estrogen receptors (ERα) immobilized on a sorbent, were assessed in terms of recovery and purification efficiency. Both approaches demonstrated their suitability to measure ultra-trace levels of estrogenic EDCs in aqueous samples. Applicability of the MIP procedure to urine and serum samples has also been demonstrated.

Keywords

Estrogenic endocrine disrupting chemicals Molecular imprinted polymer Estrogen receptors Biological matrices UHPLC-MS/MS 

Notes

Acknowledgments

The authors want to express their special thanks to all the volunteers who have permitted the collection of biological samples exploited in this work. They also thank the Centre Hospitalier Universitaire de Toulouse as the promoter of the corresponding clinical protocol.

Conflict of Interest

The authors declare no conflict of interest.

Supplementary material

216_2015_9024_MOESM1_ESM.pdf (72 kb)
ESM 1 (PDF 71 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Radia Bousoumah
    • 1
    • 2
  • Jean Philippe Antignac
    • 1
    • 3
    • 4
    Email author
  • Valérie Camel
    • 2
    • 3
    • 4
  • Marina Grimaldi
    • 5
  • Patrick Balaguer
    • 5
  • Frederique Courant
    • 6
  • Emmanuelle Bichon
    • 1
  • Marie-Line Morvan
    • 1
  • Bruno Le Bizec
    • 1
  1. 1.LUNAM Université, Oniris, USC 1329 Laboratoire d’Etude des Résidus et Contaminants dans les Aliments (LABERCA)NantesFrance
  2. 2.AgroParisTech, UMR1145 Ingénierie Procédés AlimentsParisFrance
  3. 3.INRANantesFrance
  4. 4.INRAParisFrance
  5. 5.INSERM Institut National de la santé et de la Recherche Médicale, U1194 IRCM, Institut régional du Cancer MontpellierUniversité MontpellierMontpellierFrance
  6. 6.Département Sciences de l’Environnement et Santé Publique, Faculté de PharmacieUMR HydrosciencesMontpellierFrance

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