Analytical and Bioanalytical Chemistry

, Volume 408, Issue 7, pp 1727–1733 | Cite as

Molecularly imprinted polymers with multi-functionality

Part of the following topical collections:
  1. Analytical Applications of Biomimetic Recognition Elements


Molecular imprinting is a very powerful synthetic method for preparation of robust materials with pre-designed molecular selectivity. Molecularly imprinted polymers (MIPs) are attractive substitutes for antibodies in many analytical and bioanalytical applications, e.g. for development of biosensors and for drug assays. In addition to selective molecular binding, new functions are being added to MIPs to make the synthetic materials responsive to different environmental conditions, making it possible to modulate the binding and release of different molecular targets and to simplify affinity separation. Introduction of signal-transduction functionality into MIPs also brings in new, more easily operated chemical sensors for detection and quantification of important analytical targets.

Graphical Abstract

Multi-functional molecularly imprinted polymers


Molecular imprinting Multi-functionality Nanoparticle Composite material Controlled radical polymerization 


  1. 1.
    Cheong WJ, Yang SH, Ali F (2013) Molecularly imprinted polymers for separation science: a review of reviews. J Sep Sci 36:609–628CrossRefGoogle Scholar
  2. 2.
    Ye L, Mosbach K (2008) Molecular imprinting: synthetic materials as substitutes for biological antibodies and receptors. Chem Mater 20:859–868CrossRefGoogle Scholar
  3. 3.
    Wulff G, Sarhan A (1972) The Use of polymers with enzyme-analogous structures for the resolution of racemates. Angew Chem Int Ed 11:341–341Google Scholar
  4. 4.
    Whitcombe MJ, Rodriguez ME, Villar P, Vulfson EN (1995) A new method for the introduction of recognition site functionality into polymers prepared by molecular imprinting – synthesis and characterization of polymeric receptors for cholesterol. J Am Chem Soc 117:7105–7111CrossRefGoogle Scholar
  5. 5.
    Wulff G, Knorr K (2001) Stoichiometric noncovalent interaction in molecular imprinting. Bioseparation 10:257–276CrossRefGoogle Scholar
  6. 6.
    Hall AJ, Manesiotis P, Emgenbroich M, Quaglia M, Lorenzi ED, Sellergren B (2005) Urea host monomers for stoichiometric molecular imprinting of oxyanions. J Org Chem 70:1732–1736CrossRefGoogle Scholar
  7. 7.
    Zhang H (2013) Controlled/”living” radical precipitation polymerization: a versatile polymerization technique for advanced functional polymers. Eur Polym J 49:579–600CrossRefGoogle Scholar
  8. 8.
    Alexander C, Andersson HS, Andersson LI, Ansell RJ, Kirsch N, Nicholls IA, O’Mahony J, Whitcombe MJ (2006) Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003. J Mol Recogn 19:106–180CrossRefGoogle Scholar
  9. 9.
    Whitcombe MJ, Kirsch N, Nicholls IA (2014) Molecular imprinting science and technology: a survey of the literature for the years 2004-2011. J Mol Recogn 19:106–180Google Scholar
  10. 10.
    Turkewitsch P, Wandelt B, Darling GD, Powell WS (1998) Fluorescent functional recognition sites through molecular imprinting. A polymer-based fluorescent chemosensor for aqueous cAMP. Anal Chem 70:2025–2030CrossRefGoogle Scholar
  11. 11.
    Wang W, Gao S, Wang B (1999) Building fluorescent sensors by template polymerization: the preparation of a fluorescent sensor for D-fructose. Org Lett 1:1209–1212CrossRefGoogle Scholar
  12. 12.
    Takeuchi T, Mukawa T, Shinmori H (2005) Signaling molecularly imprinted polymers: molecular recognition-based sensing materials. Chem Rec 5:263–275CrossRefGoogle Scholar
  13. 13.
    Nguyen TH, Ansell RJ (2009) Fluorescent imprinter polymer sensors for chiral amines. Org Biomed Chem 7:1211–1220CrossRefGoogle Scholar
  14. 14.
    Wagner R, Wan W, Biyikal M, Benito-Pen E, Moreno-Bondi MC, Lzraq I, Rurack K, Sellergren B (2013) Synthesis, spectroscopic, and analyte-responsive behavior of a polymerizable naphthalimide-based carboxylate probe and molecularly imprinted polymers prepared thereof. J Org Chem 78:1377–1389CrossRefGoogle Scholar
  15. 15.
    Awino JK, Zhao Y (2014) Molecularly imprinted nanoparticles as tailor-made sensors for small fluorescent molecules. Chem Commun 50:5752–5755CrossRefGoogle Scholar
  16. 16.
    Puoci F, Iemma F, Picci N (2008) Stimuli-responsive molecularly imprinted polymers for drug delivery: a review. Curr Drug Deliv 5:85–96CrossRefGoogle Scholar
  17. 17.
    Pan G, Guo Q, Cao C, Yang H, Li B (2013) Thermo-responsive molecularly imprinted nanogels for specific recognition and controlled release of proteins. Soft Mater:3840–3850Google Scholar
  18. 18.
    Fang L, Chen S, Guo X, Zhang Y, Zhang H (2012) Azobenzene-containing molecularly imprinted polymer microspheres with photo- and thermoresponsive template binding properties in pure aqueous media by atom transfer radical polymerization. Langmuir 28:9767–9777CrossRefGoogle Scholar
  19. 19.
    Takeuchi T, Sunayama H, Takano E, Kitayama Y (2015) Post-imprinting and in-cavity functionalization. Adv Biochem Eng Biotechnol. doi: 10.1007/10_2015_314 Google Scholar
  20. 20.
    Sunayama H, Takeuchi T (2014) Molecularly imprinted protein recognition cavities bearing exchangeable binding sites for postimprinting site-directed introduction of reporter molecules for readout of binding events. ACS Appl Mater Interfaces 6:20003–20009CrossRefGoogle Scholar
  21. 21.
    Haginaka J, Takehira H, Hosoya K, Tanaka N (2004) Uniform-sized molecularly imprinted polymer for (S)-naproxen selectively modified with hydrophilic external layer. J Chromatogr A 849:331–339CrossRefGoogle Scholar
  22. 22.
    Yang K, Berg MM, Zhao C, Ye L (2009) One-pot synthesis of hydrophilic molecularly imprinted nanoparticles. Macromolecules 42:8739–8746CrossRefGoogle Scholar
  23. 23.
    Moczko E, Poma A, Guerreiro A, de Vargas-Sansalvador IP, Caygill S, Canfarotta F, Whitcombe MJ, Piletsky S (2013) Surface-modified multifunctional MIP nanoparticles. Nanoscale 5:3733–3741CrossRefGoogle Scholar
  24. 24.
    Zhao M, Zhang C, Zhang Y, Guo X, Yan H, Zhang H (2014) Efficient synthesis of narrowly dispersed hydrophilic and magnetic molecularly imprinted polymer microspheres with excellent molecular recognition ability in a real biological sample. Chem Commun 50:2208–2210CrossRefGoogle Scholar
  25. 25.
    Wackerlig J, Lieberzeit PA (2015) Molecularly imprinted polymer nanoparticles in chemical sensing – synthesis, characterization and application. Sensors Actuators B 207:144–157CrossRefGoogle Scholar
  26. 26.
    Xu L, Pan J, Dai J, Cao Z, Hang H, Li X, Yan Y (2012) Magnetic ZnO surface-imprinted polymers prepared by ARGET ATRP and the application for antibiotics selective recognition. RSC Adv 2:5571–5579CrossRefGoogle Scholar
  27. 27.
    He Y, Huang Y, Jin Y, Liu X, Liu G, Zhao R (2014) Well-defined nanostructured polymers for highly selective magnetic separation of fluoroquinolones in human urine. ACS Appl Mater Interfaces 6:9634–9642CrossRefGoogle Scholar
  28. 28.
    Beyazit S, Ambrosini S, Marchyk N, Palo E, Kale V, Soukka T, Bui BTS, Haupt K (2014) Versatile synthetic strategy for coating unconverting nanoparticles with polymer shells through localized photopolymerization by using the particles as internal light sources. Angew Chem Int Ed 53:8919–8923CrossRefGoogle Scholar
  29. 29.
    Mochalin VN, Shenderova O, Ho D, Gogotsi Y (2012) The properties and applications of nanodiamonds. Nat Nanotechnol 7:11–23CrossRefGoogle Scholar
  30. 30.
    Li H, Kang Z, Liu Y, Lee S-T (2012) Carbon nanodots: synthesis, properties and applications. J Mater Chem 22:24230–24253CrossRefGoogle Scholar
  31. 31.
    Poma A, Turner APF, Piletsky SA (2010) Advances in the manufacture of MIP nanoparticles. Trends Biotechnol 28:629–637CrossRefGoogle Scholar
  32. 32.
    Rostovtsev VV, Green LG, Fokin VV, Sharpless KB (2002) A stepwise Huisgen cycloaddition process: copper(I)-catalyzed regioselective “ligation” of azides and terminal alkynes. Angew Chem Int Ed 41:2596–2599CrossRefGoogle Scholar
  33. 33.
    Liu L-H, Yan M (2010) Perfluorophenyl azides: new applications in surface functionalization and nanomaterial synthesis. Acc Chem Res 43:1434–1443CrossRefGoogle Scholar
  34. 34.
    Xu C, Ye L (2011) Clickable molecularly imprinted nanoparticles. Chem Commun 47:6096–6098CrossRefGoogle Scholar
  35. 35.
    Xu C, Shen X, Ye L (2012) Molecularly imprinted magnetic materials prepared from modular and clickable nanoparticles. J Mater Chem 22:7427–7433CrossRefGoogle Scholar
  36. 36.
    Shen X, Xu C, Uddin KMA, Larsson P-O, Ye L (2013) Molecular recognition with colloidosomes enabled by imprinted polymer nanoparticles and fluorogenic boronic acid. J Mater Chem B 1:4612–4618CrossRefGoogle Scholar
  37. 37.
    Xu C, Uddin KMA, She X, Jayawardena HSN, Yan M, Ye L (2013) Photoconjugation of molecularly imprinted polymer with magnetic nanoparticles. ACS Appl Mater Interfaces 5:5208–5213CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Division of Pure and Applied BiochemistryLund UniversityLundSweden

Personalised recommendations