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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 21, pp 6391–6404 | Cite as

Identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (Sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution Fourier-transform mass spectrometry

  • Sara Granafei
  • Ilario Losito
  • Francesco Palmisano
  • Tommaso R. I. CataldiEmail author
Research Paper
Part of the following topical collections:
  1. High-Resolution Mass Spectrometry in Food and Environmental Analysis

Abstract

The numerous and varied biological roles of phosphatidylcholines (PC) and lysophosphatidylcholines (LPC) have fueled a great demand for technologies that enable rapid, in-depth structural examination of these lipids in foodstuffs. Here, we describe the capabilities of a newly configured combination of high-efficiency liquid chromatography and high-resolution/accuracy Fourier-transform mass spectrometry with electrospray ionization (LC-ESI-FTMS), designed for lipidomics applications that require the identification of PC in their lyso forms. The devised strategy, involving a separation by hydrophilic interaction liquid chromatography (HILIC) on spherical, fused-core ultrapure silica particles (2.7 μm) of a narrow-bore column (2.1 mm i.d.), enabled the identification of as many as 71 LPC species in the lipid extracts of gilthead sea bream (Sparus aurata) fillets. In this way, LPC as proton (43) and sodium (28) adducts, i.e., [M + H]+ and [M + Na]+ ions (with M representing the zwitterionic form), were identified. In several cases, the extremely high (sub-ppm) mass accuracy and the high chromatographic efficiency available with the adopted instrumentation enabled the distinction of isobaric and closely eluting LPC species. Informative tandem mass spectra, based on high-energy collision induced dissociation (HCD), were also obtained, thus distinguishing regioisomeric LPC species (i.e., LPC differing only for the location of the residual side chain on the glycerol backbone) and between proton and sodium adducts.

Graphical Abstract

Extracted Ion Current chromatogram (XIC) obtained for the m/z value 568.339, showing the presence of two regioisomeric Lysophosphatidylcholines. The corresponding high collisional energy tandem MS spectra, obtained using a HCD cell, are shown as insets

Keywords

Seafood lipidomics Lysophospholipids High-resolution MS Orbitrap MS Hydrophilic interaction liquid chromatography Sparus aurata 

Notes

Acknowledgments

This work was supported by the project PONa3_00395/1 “BIOSCIENZE & SALUTE (B&H)” of the Italian Ministero per l'Istruzione, l'Università e la Ricerca (MIUR), and through the research project PRIN 2012TLC44W_003. The authors wish to thank the S.C.A. s.r.l. (Servizi Chimici Ambientali) laboratory for the ICP-MS analyses.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sara Granafei
    • 1
  • Ilario Losito
    • 1
    • 2
  • Francesco Palmisano
    • 1
    • 2
  • Tommaso R. I. Cataldi
    • 1
    • 2
    Email author
  1. 1.Dipartimento di ChimicaUniversità degli Studi di Bari Aldo MoroBariItaly
  2. 2.Centro di Ricerca Interdipartimentale S.M.A.R.T.Università degli Studi di Bari Aldo MoroBariItaly

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