Analytical and Bioanalytical Chemistry

, Volume 407, Issue 16, pp 4829–4834 | Cite as

GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays

  • Cong Wang
  • Rong Li
  • Sheng Quan
  • Ping Shen
  • Dabing Zhang
  • Jianxin Shi
  • Litao Yang
Note

Abstract

Isothermal DNA/RNA amplification techniques are the primary methodology for developing on-spot rapid nucleic acid amplification assays, and the loop-mediated isothermal amplification (LAMP) technique has been developed and applied in the detection of foodborne pathogens, plant/animal viruses, and genetically modified (GM) food/feed contents. In this study, one set of LAMP assays targeting on eight frequently used universal elements, marker genes, and exogenous target genes, such as CaMV35S promoter, FMV35S promoter, NOS, bar, cry1Ac, CP4 epsps, pat, and NptII, were developed for visual screening of GM contents in plant-derived food samples with high efficiency and accuracy. For these eight LAMP assays, their specificity was evaluated by testing commercial GM plant events and their limits of detection were also determined, which are 10 haploid genome equivalents (HGE) for FMV35S promoter, cry1Ac, and pat assays, as well as five HGE for CaMV35S promoter, bar, NOS terminator, CP4 epsps, and NptII assays. The screening applicability of these LAMP assays was further validated successfully using practical canola, soybean, and maize samples. The results suggested that the established visual LAMP assays are applicable and cost-effective for GM screening in plant-derived food samples.

Keywords

Genetically modified organism Loop-mediated isothermal amplification Screening test 

Supplementary material

216_2015_8652_MOESM1_ESM.pdf (977 kb)
ESM 1(PDF 976 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Cong Wang
    • 1
  • Rong Li
    • 1
  • Sheng Quan
    • 1
  • Ping Shen
    • 2
  • Dabing Zhang
    • 1
  • Jianxin Shi
    • 1
  • Litao Yang
    • 1
  1. 1.Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Development Center of Science and TechnologyMinistry of Agriculture of People’s Republic of ChinaBeijingChina

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