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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 16, pp 4823–4827 | Cite as

Quantification of 3-MCPD and its mercapturic metabolite in human urine: validation of an LC–MS–MS method and its application in the general population

  • Roberta AndreoliEmail author
  • Martina Cirlini
  • Antonio Mutti
Note

Abstract

A new method for the simultaneous quantitative determination in human urine of 3-monochloropropane-1,2-diol (3-MCPD), a toxic food contaminant, and its metabolite, 2,3-dihydroxypropyl mercapturic acid (DHPMA), was developed and validated. After urine dilution, the analytes were separated on an Atlantis®dC18 column and quantified by liquid chromatography–tandem mass spectrometry using isotopically labelled internal standards. The limits of quantification (S/N = 10) were 1.90 and 2.21 μg/L for 3-MCPD and DHPMA, respectively. Intra- and inter-day precision were lower than 6 % for each compound. Matrix effects were evaluated. Due to the high sensitivity and good accuracy of the method, 3-MCPD and DHPMA were found in 67 and 100 % of urine samples of healthy subjects, respectively.

Keywords

3-MCPD DHPMA Human urine LC–MS–MS 

Notes

Acknowledgments

This study was partially supported by the European Food Safety Authority (EFSA) within the project named “Comparison between 3-MCPD and its palmitic esters in a 90-day toxicological study.”

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Roberta Andreoli
    • 1
    Email author
  • Martina Cirlini
    • 2
  • Antonio Mutti
    • 1
  1. 1.Laboratory of Industrial Toxicology, Department of Clinical and Experimental MedicineUniversity of ParmaParmaItaly
  2. 2.Department of Food ScienceUniversity of ParmaParmaItaly

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