Development of an enzyme-linked-immunosorbent-assay technique for accurate identification of poorly preserved silks unearthed in ancient tombs
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We report the preparation of a specific fibroin antibody and its use for the identification of unearthed ancient silk relics. Based on the 12-amino-acid repeat sequence “GAGAGSGAGAGS”, which is found in fibroin of the silkworm Bombyx mori, a specific antibody against fibroin was prepared in rabbits through peptide synthesis and carrier-protein coupling. This antibody was highly specific for fibroin found in silk. Using this antibody we have successfully identified four silk samples from different time periods. Our results reveal, for the first time, a method capable of detecting silk from a few milligrams of archaeological fabric that has been buried for thousands of years, confirming that the ancient practice of wearing silk products while praying for rebirth dated back to at least 400 BCE. This method also complements current approaches in silk detection, especially for the characterization of poorly preserved silks, promoting the investigation of silk origins and of ancient clothing cultures.
KeywordsFibroin antibody Archaeological fabric ELISA Identification Silk
We thank the Materials and Textiles Institute of Zhejiang Sci-Tech University (Hangzhou, China) for providing the modern fiber samples. This work was supported by the grant of the State Administration of Cultural Heritage, project 20120226 on Study of Silk Relics by Enzyme-linked Immunosorbent Assay and the grant of the National Key Technology R&D Program, project 2013BAK08B08 on Identification and Conservation of Archaeological Remains.
- 4.Kim J, Wyeth P (2009) Preservation 6:60–67Google Scholar
- 5.Kim J, Zhang X, Wyeth P (2008) E-ps 5:41–46Google Scholar
- 8.Cattaneo C, Gelsthorpe K, Phillips P, Sokol RJ, Smillie D (1991) Antiquity 65:878–881Google Scholar
- 11.Yan F, Ge QY, Li Q, Yu M, Zhu XD, Pan J (2014) Sci Conserv Archaeol 26:71–75Google Scholar
- 12.Zhang K, Zhang BJ, Fang SQ (2013) Sci Conserv Archaeol 25:94–102Google Scholar
- 13.Zhou Y, Wang B, Sui M, Zhao F, Hu Z (2014) Studies in ConservationGoogle Scholar
- 26.Zhao F (1996) Southeast Cult 1:67–74Google Scholar