Analytical and Bioanalytical Chemistry

, Volume 407, Issue 12, pp 3471–3479 | Cite as

Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products

  • Eric NuritEmail author
  • Bernard Lyan
  • Agnès Piquet
  • Gérard Branlard
  • Estelle Pujos-Guillot
Research Paper


Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.

Graphical Abstract

Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread


LC-MS/MS Water-soluble vitamins Food processing Wheat 

Supplementary material

216_2015_8553_MOESM1_ESM.pdf (23 kb)
ESM 1 (PDF 23 kb)


  1. 1.
    Fardet A (2010) New hypotheses for the health protective mechanisms of whole grain cereals: what is beyond fiber? Nutr Res Rev 23:65–134CrossRefGoogle Scholar
  2. 2.
    Blake CJ (2007) Analytical procedures for water-soluble vitamins in foods and dietary supplements: a review. Anal Bioanal Chem 389:63–76CrossRefGoogle Scholar
  3. 3.
    Ball GFM (2006) Vitamins in foods, analysis, bioavailability and stability. CRC, LondonGoogle Scholar
  4. 4.
    Rizzolo A, Polesello S (1992) Chromatographic determination of vitamins in foods. J Chromatogr A 624:103–152CrossRefGoogle Scholar
  5. 5.
    Trang HK (2013) Development of HPLC methods for the determination of water-soluble vitamins in pharmaceuticals and fortified food products. All Theses. Paper 1745Google Scholar
  6. 6.
    Langer S, Lodge JK (2014) Determination of selected water-soluble vitamins using hydrophilic chromatography: a comparison of photodiode array, fluorescence, and coulometric detection, and validation in a breakfast cereal matrix. J Chromatogr B 960:73–81CrossRefGoogle Scholar
  7. 7.
    Gentili A, Caretti F, D’Ascenzo G, Marchese S, Perret D, Di Corcia D, Mainero Rocca L (2008) Simultaneous determination of water-soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 22:2029–2043CrossRefGoogle Scholar
  8. 8.
    Leporati A, Catellania D, Sumana M, Andreolib R, Maninib P, Niessenc WMA (2005) Application of a liquid chromatography tandem mass spectrometry method to the analysis of water-soluble vitamins in Italian pasta. Anal Chim Acta 531:87–95CrossRefGoogle Scholar
  9. 9.
    Baiyi L, Yiping R, Baifen H, Wenquin L, Zengxuan C, Xiaowei T (2008) Simultaneous determination of four water-soluble vitamins in fortified foods by ultra-performance liquid chromatography couple with triple quadrupole mass spectrometry. J Chromatogr Sci 46:225–231CrossRefGoogle Scholar
  10. 10.
    Ndaw S, Bergaentzlé M, Aoudé-Werner D, Hasselmann C (2000) Extraction procedures for the liquid chromatographic determination of thiamin, riboflavin and vitamin B6 in foodstuffs. Food Chem 71:129–138CrossRefGoogle Scholar
  11. 11.
    Gratacos-Cubrasi M, Sarraga C, Clariana M, Garcia Regueiro JA, Castellari M (2011) Analysis of vitamin B1 in dry-cured sausages by hydrophilic interaction liquid chromatography (HILIC) and diode detection. Meat Sci 87:234–238CrossRefGoogle Scholar
  12. 12.
    Karatapanis E, Fiamegos C, Stalikas D (2009) HILIC separation and quantitation of water-soluble vitamins using diol column. J Sep Sci 32:909–917CrossRefGoogle Scholar
  13. 13.
    Ndaw S, Bergaentzlé M, Aoudé-Werner D, Hasselmann C (2002) Enzymatic extraction procedure for the liquid chromatographic determination of niacin in foodstuffs. Food Chem 78:129–134CrossRefGoogle Scholar
  14. 14.
    Taylor PJ (2005) Matrix effects: the Achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry. Clin Biochem 38:328–334CrossRefGoogle Scholar
  15. 15.
    Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal Chem 79:3019–30CrossRefGoogle Scholar
  16. 16.
    Matuszewski BK (2006) Standard line slopes as a measure of a relative matrix effect in quantitative HPLC-MS bioanalysis. J Chromatogr B 830:293–300CrossRefGoogle Scholar
  17. 17.
    Batifoulier F, Verny MA, Chanliaud E, Remesy C (2005) Effect of different breadmaking methods on thiamin, riboflavine and pyridoxine contents of wheat bread. J Cereal Sci 42:101–108CrossRefGoogle Scholar
  18. 18.
    Pederson B, Knudsen KEB, Eggum BO (1989) Nutritive value of cereal products with emphasis on the effect of milling. World Rev Nutr Diet 60:1–5Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Eric Nurit
    • 3
    Email author
  • Bernard Lyan
    • 1
  • Agnès Piquet
    • 2
  • Gérard Branlard
    • 3
  • Estelle Pujos-Guillot
    • 1
  1. 1.INRA, UMR1019, Plateforme d’Exploration du Métabolisme, UNHClermont-FerrandFrance
  2. 2.VetagroSup, Campus Agronomique de ClermontLempdesFrance
  3. 3.INRA, UMR 1095, Génétique, Diversité et écophysiologie des céréalesClermont-FerrandFrance

Personalised recommendations