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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 12, pp 3471–3479 | Cite as

Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products

  • Eric NuritEmail author
  • Bernard Lyan
  • Agnès Piquet
  • Gérard Branlard
  • Estelle Pujos-Guillot
Research Paper

Abstract

Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.

Graphical Abstract

Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread

Keywords

LC-MS/MS Water-soluble vitamins Food processing Wheat 

Supplementary material

216_2015_8553_MOESM1_ESM.pdf (23 kb)
ESM 1 (PDF 23 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Eric Nurit
    • 3
    Email author
  • Bernard Lyan
    • 1
  • Agnès Piquet
    • 2
  • Gérard Branlard
    • 3
  • Estelle Pujos-Guillot
    • 1
  1. 1.INRA, UMR1019, Plateforme d’Exploration du Métabolisme, UNHClermont-FerrandFrance
  2. 2.VetagroSup, Campus Agronomique de ClermontLempdesFrance
  3. 3.INRA, UMR 1095, Génétique, Diversité et écophysiologie des céréalesClermont-FerrandFrance

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