Analytical and Bioanalytical Chemistry

, Volume 407, Issue 12, pp 3525–3529 | Cite as

Synthesis of boronic-acid-functionalized magnetic attapulgite for selective enrichment of nucleosides

  • Ting Cheng
  • Huihui Li
  • Ying Ma
  • Xiaoyan Liu
  • Haixia ZhangEmail author


2,4-Difluoro-3-formyl-phenylboronic acid (DFFPBA)-modified magnetic attapulgite (ATP-Fe3O4-NH2-DFFPBA) was synthesized and employed to capture and enrich cis-diol-containing biomolecules. The resulting material exhibited a high saturation magnetization value of 20.71 emu/g, allowing the absorbent to be conveniently magnetically separated. Combining the Fe3O4 nanoparticles with the high specific surface area of attapulgite yielded a material with a high capture capacity (13.78 mg/g) for adenosine. Furthermore, ATP-Fe3O4-NH2-DFFPBA was found to possess remarkable selectivity for adenosine at a low molar ratio of adenosine/2-deoxyadenosine (1:500). The potential applications of this material were explored by using it to extract five nucleosides from urine samples, and the results demonstrate that it can decrease matrix interference and selectively enrich analytes.

Graphical Abstract

Boronic-acid-functionalized magnetic attapulgite could selectively enrich the nucleosides in urine samples with the help of an external magnet


Boronate affinity chromatography Nucleosides Magnetic Attapulgite Enrichment 



The authors thank the support provided by the National Science Foundation of China (nos. 21375052 and J1103307).

Supplementary material

216_2015_8550_MOESM1_ESM.pdf (407 kb)
ESM 1 (PDF 407 kb)


  1. 1.
    James TD, Sandanayake KRAS, Shinka S (1996) Saccharide sensing with molecular receptors based on boronic acid. Angew Chem Int Ed Engl 35:1910–1922CrossRefGoogle Scholar
  2. 2.
    Chen M, Lu Y, Ma Q, Guo L, Feng YQ (2009) Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins. Analyst 134:2158–21643CrossRefGoogle Scholar
  3. 3.
    Xu Y, Wu Z, Zhang L, Lu H, Yang P, Webley PA, Zhao D (2009) Highly specific enrichment of glycopeptides using boronic acid-functionalized mesoporous silica. Anal Chem 81:503–508CrossRefGoogle Scholar
  4. 4.
    Li H, Shan Y, Qiao L, Dou A, Shi X, Xu G (2013) Facile synthesis of boronate-decorated polyethyleneimine-grafted hybrid magnetic nanoparticles for the highly selective enrichment of modified nucleosides and ribosylated metabolites. Anal Chem 85:11585–11592CrossRefGoogle Scholar
  5. 5.
    Li QJ, Lü CC, Liu Z (2013) Preparation and characterization of fluorophenylboronic acid functionalized monolithic columns for high affinity capture of cis-diol containing compounds. J Chromatogr A 1305:123–130Google Scholar
  6. 6.
    Liu YS, Liu P, Su ZX, Li FS, Wen FS (2008) Attapulgite-Fe3O4 magnetic nanoparticles via co-precipitation technique. Appl Surf Sci 255:2020–2025CrossRefGoogle Scholar
  7. 7.
    Rodriguez-Gonzalo E, Hernandez-Prieto R, Garcia-Gomez D, Carabias-Martinez R (2013) Capillary electrophoresis–mass spectrometry for direct determination of urinary modified nucleosides. Evaluation of synthetic urine as a surrogate matrix for quantitative analysis. J Chromatogr B 942:21–30CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ting Cheng
    • 1
  • Huihui Li
    • 1
  • Ying Ma
    • 1
  • Xiaoyan Liu
    • 1
  • Haixia Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceLanzhou UniversityLanzhouChina

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