Advertisement

Analytical and Bioanalytical Chemistry

, Volume 407, Issue 15, pp 4343–4349 | Cite as

Clinical biochemical and hormonal profiling in plasma: a promising strategy to predict growth hormone abuse in cattle

  • Mickael Doué
  • Gaud Dervilly-Pinel
  • Nora Cesbron
  • Annalisa Stefani
  • Letizia Moro
  • Giancarlo Biancotto
  • Bruno Le Bizec
Rapid Communication
Part of the following topical collections:
  1. Hormone and Veterinary Drug Residue Analysis

Abstract

Recombinant bovine somatotrophin (rbST) is widely used in some countries to increase milk production. Since 1994, both marketing and use of this substance have been prohibited within the European Union. In this context, the targeted plasma biochemical and hormonal profiling was assessed as a potential screening strategy to highlight rbST (ab)use in cattle. Twenty-one routinely measured clinical blood parameters, representative of main biological profiles (energetic, proteic, etc.), were measured in the plasma of six lactating cows before and after rbST treatment throughout a 23-day study period. Appropriate multivariate statistical analyses [principal component analysis (PCA) and orthogonal partial least square (OPLS)] enabled discriminating animal samples before and after treatment (days 0 vs. 2 to 9, P = 2.10−9) and highlighted the five most relevant blood parameters in this discrimination. Based on each five-analyte contribution, a simple mathematically weighted equation was suggested to predict the status of samples. A suspicious threshold was proposed, and the model was further tested with the status prediction of the supplementary samples from untreated (n = 20) and treated cows (n = 22). The calculated false-positive (10 %) and false-negative (4.5 %) rates were in accordance with the EU requirements for screening methods. Although the model needs to be further validated with additional samples, such targeted plasma biochemical and hormonal profiling already appears as a potential promising screening strategy to highlight rbST (ab)use in cattle.

Keywords

Bovine recombinant somatotrophin Blood parameters Screening method Biomarkers 

Notes

Acknowledgments

Mickael Doué was a fellowship recipient of the French Region Pays de la Loire and the French Ministry of Agriculture (contract no. 2011-09551).

References

  1. 1.
    Etherton TD, Bauman DE (1998) Biology of somatotropin in growth and lactation of domestic animals. Physiol Rev 78:745–761Google Scholar
  2. 2.
    Bauman DE (1999) Bovine somatotropin and lactation: from basic science to commercial application. Domest Anim Endocrinol 17:101–116CrossRefGoogle Scholar
  3. 3.
    European Council Decision of 17 December 1999 concerning the placing on the market and administration of bovine somatotropin (bST) and repealing Decision 90/128/EC, Off J Eur Commun. OJ no. L 331, p. 71Google Scholar
  4. 4.
    Dervilly-Pinel G, Prévost S, Monteau F, Le Bizec B (2014) Analytical strategies to detect use of recombinant bovine somatotropin in food-producing animals. TrAC Trends Anal Chem 53:1–10CrossRefGoogle Scholar
  5. 5.
    Le Breton MH, Rochereau-Roulet S, Pinel G, Cesbron N, Le Bizec B (2009) Elimination kinetic of recombinant somatotropin in bovine. Anal Chim Acta 637:121–127CrossRefGoogle Scholar
  6. 6.
    European Commission Decision of 12 August 2002 implemented Council Directive 96/23/EC concerning the performance of analytical methods and interpretation of results. Off J Eur Commun, 2002/657/EC. OJ no. L 221, p. 8Google Scholar
  7. 7.
    Rochereau-Roulet S, Gaudin I, Chéreau S, Prévost S, André-Fontaine G, Pinel G, Le Bizec B (2011) Development and validation of an enzyme-linked immunosorbent assay for the detection of circulating antibodies raised against growth hormone as a consequence of rbST treatment in cows. Anal Chim Acta 700:189–193CrossRefGoogle Scholar
  8. 8.
    Ludwig SKJ, Smits NGE, van der Veer G, Bremer MGEG, Nielen MWF (2012) Multiple protein biomarker assessment for recombinant bovine somatotropin (rbST) abuse in Cattle. PLoS ONE 7:e52917CrossRefGoogle Scholar
  9. 9.
    Doornenbal H, Tong AKW, Murray NL (1988) Reference values of blood parameters in beef cattle of different ages and stages of lactation. Can J Vet Res 52:99–105Google Scholar
  10. 10.
    Cozzi G, Ravarotto L, Gottardo F, Stefani AL, Contiero B, Moro L, Brscic M, Dalvit P (2011) Short communication: reference values for blood parameters in Holstein dairy cows: effects of parity, stage of lactation, and season of production. J Dairy Sci 94:3895–3901CrossRefGoogle Scholar
  11. 11.
    Groot MJ (2002) Hepatitis in growth promoter treated cows. J Vet Med A 49:466–469CrossRefGoogle Scholar
  12. 12.
    Marin A, Pozza G, Gottardo F, Moro L, Stefani AL, Cozzi G, Brscic M, Andrighetto I, Ravarotto L (2008) Administration of dexamethasone per os in finishing bulls. II. Effects on blood parameters used as indicators of animal welfare. Animal 2:1080–1086CrossRefGoogle Scholar
  13. 13.
    Cunningham RT, Mooney MH, Xia XL, Crooks S, Matthews D, O’Keeffe M, Li K, Elliott CT (2009) Feasibility of a clinical chemical analysis approach to predict misuse of growth promoting hormones in Cattle. Anal Chem 81:977–983CrossRefGoogle Scholar
  14. 14.
    Smith BP (2008) In: Mosby (ed) Large animal internal medicine. Mosby Elsevier, St Louis, p 1381Google Scholar
  15. 15.
    Soliman EB, EL-Barody MAA (2014) Physiological responses of dairy animals to recombinant bovine somatotropin: a review. J Cell Anim Biol 8:1–14CrossRefGoogle Scholar
  16. 16.
    Eriksson L, Trygg J, Wold S (2008) CV-ANOVA for significance testing of PLS and OPLS® models. J Chemom 22:594–600CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Mickael Doué
    • 1
  • Gaud Dervilly-Pinel
    • 1
  • Nora Cesbron
    • 1
    • 2
  • Annalisa Stefani
    • 3
  • Letizia Moro
    • 3
  • Giancarlo Biancotto
    • 3
  • Bruno Le Bizec
    • 1
  1. 1.Oniris, Laboratoire d’Etude des Résidus et Contaminants dans les Aliments (LABERCA)LUNAM UniversitéNantesFrance
  2. 2.OnirisCentre Hospitalier Universitaire VeterinaireNantesFrance
  3. 3.Istituto Zooprofilattico Sperimentale delle VenezieLegnaroItaly

Personalised recommendations