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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 15, pp 4411–4421 | Cite as

High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry

  • A. RúbiesEmail author
  • G. Sans
  • P. Kumar
  • M. Granados
  • R. Companyó
  • F. Centrich
Research Paper
Part of the following topical collections:
  1. Hormone and Veterinary Drug Residue Analysis

Abstract

We present a high-throughput confirmatory method for the analysis of 11 nitroimidazoles in muscle samples (metronidazole, MNZ; dimetridazole, DMZ; ronidazole, RNZ; tinidazole, TNZ; carnidazole, CRZ; secnidazole semihydrate, SCZ; ornidazole, ORZ; metronidazole-hydroxy, MNZ-OH; ipronidazole, IPZ; ipronidazole-hydroxy, IPZ-OH; and dimetridazole-hydroxy, HMMNI). Extraction with acetonitrile is efficient and simple, with the majority of recoveries ranging between 65 and 101 %, and without the need for clean-up of the extracts. The chromatographic analysis is performed using UPLC-QqQ-MS in the MRM mode, with an electrospray source operated in positive mode. Total chromatographic analysis time is 12 min. This method has been fully validated in muscle according to Decision 657/2002/CE guidelines, and the CCα values obtained were in the range of 0.04–0.4 μg·kg−1. The method is currently used for the analysis of muscle samples and has been tested in other matrices of animal origin, such as kidney, retina and egg, with excellent results.

Keywords

Liquid chromatography Mass spectrometry Triple quadrupole Nitroimidazoles Food samples 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • A. Rúbies
    • 1
    • 3
    Email author
  • G. Sans
    • 1
    • 2
  • P. Kumar
    • 1
    • 2
  • M. Granados
    • 2
  • R. Companyó
    • 2
  • F. Centrich
    • 1
    • 3
  1. 1.Laboratori de l’Agència de Salut Pública de BarcelonaBarcelonaSpain
  2. 2.Department of Analytical ChemistryUniversity of BarcelonaBarcelonaSpain
  3. 3.CIBER of Epidemiology and Public Health (CIBERESP)MadridSpain

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